Document Detail


Inhibitory effect of disodium EDTA upon the growth of Staphylococcus epidermidis in vitro: relation to infection prophylaxis of Hickman catheters.
MedLine Citation:
PMID:  3150914     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Granulocytopenic patients with an intravascular catheter are at increased risk for infection with Staphylococcus epidermidis. During the intervals when the catheters are not being used for infusions, it is customary to maintain patency of the catheter lumen with a solution containing heparin. We show that heparin does not inhibit the growth of S. epidermidis isolated from the catheter of an infected patient. A 20-mg/ml solution of disodium EDTA, a chelating agent which effectively anticoagulates blood at this concentration, was shown to be bactericidal for an initial inoculum of 10(3) CFU of staphylococci per ml in 24 h. Vancomycin, an antibiotic which is often employed to treat Staphylococcus infections, was also bactericidal for initial inocula of 10(3) CFU/ml at doses of 6.7 micrograms/ml, a drug concentration in the therapeutic range. When 10(3) staphylococci per ml were cultured in the presence of catheter segments and disodium EDTA or vancomycin, subcultures of the catheters showed minimal or no growth, respectively. In contrast, when cultured with heparin alone, subcultures showed abundant growth. In view of its low cost, effectiveness as an anticoagulant, and bactericidal activity, EDTA should be studied as a replacement for heparin solutions for the maintenance of intravenous catheters in granulocytopenic patients.
Authors:
J L Root; O R McIntyre; N J Jacobs; C P Daghlian
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Antimicrobial agents and chemotherapy     Volume:  32     ISSN:  0066-4804     ISO Abbreviation:  Antimicrob. Agents Chemother.     Publication Date:  1988 Nov 
Date Detail:
Created Date:  1989-08-09     Completed Date:  1989-08-09     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0315061     Medline TA:  Antimicrob Agents Chemother     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1627-31     Citation Subset:  IM    
Affiliation:
Department of Medicine, Dartmouth-Hitchcock Medical Center, Hanover, New Hampshire.
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MeSH Terms
Descriptor/Qualifier:
Catheters, Indwelling / adverse effects*
Edetic Acid / pharmacology*
Heparin / pharmacology
Humans
Microbial Sensitivity Tests
Staphylococcal Infections / prevention & control*
Staphylococcus epidermidis / drug effects*,  growth & development
Vancomycin / pharmacology
Grant Support
ID/Acronym/Agency:
CA19379-11/CA/NCI NIH HHS; CA23108-09/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
1404-90-6/Vancomycin; 60-00-4/Edetic Acid; 9005-49-6/Heparin
Comments/Corrections

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