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Inhibitory effect of curcumin on the invasion of rat ascites hepatoma cells in vitro and ex vivo.
MedLine Citation:
PMID:  19003281     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Curcumin, a yellow pigment in turmeric, is a food factor withantioxidative activity. The effect of curcumin on the proliferation and invasion of the rat ascites hepatoma AH109Acells was studied in vitro and ex vivo assay systems. Especially, a co-culture system of the hepatoma cellswith mesothelial cells derived from rat mesentery was employed to investigate the invasive motility. Curcumin suppressed thehepatoma slipping motility in a dose-dependent manner up to 5 muM and thereafter maintained the effect up to 20 muM, whereas this substance exerted little influence on the proliferation of the hepatoma cells at the same concentrations. Sera obtained from rats orally given curcumin also inhibited the AH109A cellular invasive movement when added to the culturemedium. Hepatoma cells previously cultured with hypoxanthineand xanthine oxidase showed a highly invasive activity. Curcumin and curcumin-loaded rat sera suppressed this reactive oxygen species-potentiated invasive capacity by simultaneously treating AH109A cells with hypoxanthine, xanthine oxidase and either of curcumin samples. These resultssuggest that the antioxidative property of curcumin may beinvolved in its anti-invasive action.
Authors:
Y Kozuki; Y Miura; K Yagasaki
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Cytotechnology     Volume:  35     ISSN:  0920-9069     ISO Abbreviation:  Cytotechnology     Publication Date:  2001 Jan 
Date Detail:
Created Date:  2008-11-12     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8807027     Medline TA:  Cytotechnology     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  57-63     Citation Subset:  -    
Affiliation:
Department of Applied Biological Science, Tokyo Noko University, Fuchu, Tokyo, 183-8509, Japan.
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