| Inhibitory effect of apoptosis in human astrocytes CCF-STTG1 cells by lemon oil. | |
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MedLine Citation:
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PMID: 12162947 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The effects of lemon pure essential oils on the heat shock-induced apoptosis in human astrocytes cell line CCF-STTG1 were examined. In previous studies, heat shock has been reported to induce the apoptosis or programmed cell death through the activation of caspase-3. Treatment of heat shock on CCF-STTG1 cells markedly induced apoptotic cell death as determined by flow cytometry. Interestingly, pre-treatment with lemon pure essential oils on CCF-STTG1 cells inhibited the heat shock-induced apoptosis. Lemon oil also inhibited the heat shock-induced apoptosis in primary cultured rat astrocytes. To determine whether lemon oil inhibits the heat shock-induced activation of the apoptotic proteases, activation of caspase-3 was assessed by Western blotting. DNA fragmentation, giemsa staining, and heat shock-induced activation of caspase-3 were blocked by lemon pure essential oil, which is consistent with flow cytometry. Poly-ADP-ribose polymerase (PARP), the cysteine protease substrate, was fragmented as a consequence of apoptosis by heat shock. Lemon oil inhibited the PARP fragmentation. These results suggest that lemon pure essential oils may modulate the apoptosis through the activation of the interleukin-1 beta -converting enzyme-like caspases. |
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Authors:
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Hyun-Na Koo; Seung-Heon Hong; Chae-Yun Kim; Jong-Woong Ahn; Yong-Geun Lee; Jae-Ju Kim; Yeung-Su Lyu; Hyung-Min Kim |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Pharmacological research : the official journal of the Italian Pharmacological Society Volume: 45 ISSN: 1043-6618 ISO Abbreviation: Pharmacol. Res. Publication Date: 2002 Jun |
Date Detail:
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Created Date: 2002-08-06 Completed Date: 2003-04-02 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 8907422 Medline TA: Pharmacol Res Country: England |
Other Details:
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Languages: eng Pagination: 469-73 Citation Subset: IM |
Affiliation:
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Department of Oriental Pharmacy, College of Pharmacy, Wonkwang University, KI Co. Ltd. Iksan, Chonbuk 570-749, Republic of Korea. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Apoptosis / drug effects* Astrocytes / cytology, drug effects, enzymology* Blotting, Western Brain / cytology, enzymology Caspase 3 Caspases / metabolism Cell Line Cell Nucleus / metabolism, ultrastructure Chromatin / metabolism Citrus / chemistry* DNA / biosynthesis, chemistry DNA Fragmentation / drug effects Depression, Chemical Electrophoresis, Agar Gel Enzyme Activation Flow Cytometry Hot Temperature* Humans Plant Oils / pharmacology* Poly(ADP-ribose) Polymerases / chemistry, metabolism Rats |
| Chemical | |
Reg. No./Substance:
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0/Chromatin; 0/Plant Oils; 9007-49-2/DNA; EC 2.4.2.30/Poly(ADP-ribose) Polymerases; EC 3.4.22.-/CASP3 protein, human; EC 3.4.22.-/Casp3 protein, rat; EC 3.4.22.-/Caspase 3; EC 3.4.22.-/Caspases |
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