Document Detail

Inhibitory effect of anoxia on 125I-insulin binding by rat hepatocytes.
MedLine Citation:
PMID:  3522146     Owner:  NLM     Status:  MEDLINE    
We have examined the possibility that 125I-insulin binding by isolated rat hepatocytes is modulated by cellular ATP levels. To avoid complications due to ATP-dependent internalization of bound insulin, 125I-insulin binding was determined at 10 degrees C; at this temperature, equilibrium binding was achieved after incubation for 4-6 h. When hepatocytes were incubated at 37 degrees C under anaerobic conditions, ATP levels and 125I-insulin binding were both lowered by about 65%. Anoxia inhibited the association of 125I-insulin with the hepatocyte receptor; the dissociation of insulin from hepatocytes was not affected. Cellular ATP levels and 125I-insulin binding were both restored when anaerobic cells were incubated further at 37 degrees C under aerobic conditions. When anaerobic cells were incubated in air at 10 degrees C during the insulin binding assay, 125I-insulin binding recovered completely, but ATP levels were unaffected. The inhibitory effect of anoxia on 125I-insulin binding was not due to any effect on 125I-insulin degradation or on cell viability. We conclude (1) that the ability of hepatocytes to bind insulin can be modulated on a short-term basis in response to the metabolic status of the cell, and (2) that modulation of the liver cell insulin receptor is not a function of cellular ATP levels.
S M Kelemen; H Reid; M K Gould
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Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  Diabetes research and clinical practice     Volume:  2     ISSN:  0168-8227     ISO Abbreviation:  Diabetes Res. Clin. Pract.     Publication Date:  1986 Apr 
Date Detail:
Created Date:  1986-07-28     Completed Date:  1986-07-28     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  8508335     Medline TA:  Diabetes Res Clin Pract     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  15-22     Citation Subset:  IM    
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MeSH Terms
Insulin / metabolism*
Iodine Radioisotopes
Liver / metabolism*
Oxygen / physiology*
Receptor, Insulin / metabolism
Reg. No./Substance:
0/Iodine Radioisotopes; 11061-68-0/Insulin; 7782-44-7/Oxygen; EC, Insulin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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