Document Detail

Inhibitory effect of acetylshikonin on human gastric carcinoma cell line SGC-7901 in vitro and in vivo.
MedLine Citation:
PMID:  19370777     Owner:  NLM     Status:  MEDLINE    
AIM: To investigate the inhibitory effect of acetylshikonin on human gastric carcinoma cell line SGC-7901 and its mechanism.
METHODS: MTT assay was used to assess the inhibitory effect of acetylshikonin on proliferation of SGC-7901 cells. Apoptosis-inducing effect was determined by flow cytometry and terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end-labeling with Hoechst staining. Expression of mRNA and protein in Bcl-2 and Bax was analyzed by reverse transcription-polymerase chain reaction and Western blot. Antitumor effect of acetylshikonin on a mouse SGC-7901 model was also determined.
RESULTS: Forty-eight hours after treatment with acetylshikonin, MTT assay showed that acetylshikonin inhibited the proliferation of SGC-7901 cells in a dose-dependent manner. The half maximal inhibitory concentration of acetylshikonin to SGC-7901 cells was 0.428 +/- 0.07 mg/L. Cell shrinkage, nuclear pyknosis and chromatin condensation, which are the characteristics of cell apoptosis, were observed in treated SGC-7901 cells and the percentage of apoptosis increased in a dose-dependent manner. Acetylshikonin down-regulated the expression of Bcl-2 and up-regulated the expression of Bax in the treated SGC-7901 cells compared with the controls. The experiment in vivo showed that 0.5, 1, and 2 mg/kg of acetylshikonin significantly inhibited the growth of tumor in the mouse SGC-7901 model, with an inhibitory rate of 25.00%-55.76%.
CONCLUSION: Acetylshikonin inhibits the growth of SGC-7901 cells in vitro and in vivo by inducing cell apoptosis.
Yun Zeng; Gang Liu; Li-Ming Zhou
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  World journal of gastroenterology : WJG     Volume:  15     ISSN:  2219-2840     ISO Abbreviation:  World J. Gastroenterol.     Publication Date:  2009 Apr 
Date Detail:
Created Date:  2009-04-16     Completed Date:  2009-05-26     Revised Date:  2014-05-20    
Medline Journal Info:
Nlm Unique ID:  100883448     Medline TA:  World J Gastroenterol     Country:  China    
Other Details:
Languages:  eng     Pagination:  1816-20     Citation Subset:  IM    
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MeSH Terms
Anthraquinones / pharmacology*
Caspase 3 / metabolism
Cell Cycle / drug effects
Cell Line, Tumor / drug effects*,  metabolism
Cell Proliferation / drug effects
Drugs, Chinese Herbal / pharmacology*
Mice, Nude
Neoplasm Transplantation
Proto-Oncogene Proteins c-bcl-2 / genetics,  metabolism
Stomach Neoplasms* / metabolism,  pathology
bcl-2-Associated X Protein / genetics,  metabolism
Reg. No./Substance:
0/Anthraquinones; 0/Drugs, Chinese Herbal; 0/Proto-Oncogene Proteins c-bcl-2; 0/bcl-2-Associated X Protein; 54984-93-9/acetylshikonin; EC 3.4.22.-/Caspase 3

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