| Inhibitory effect of acetylshikonin on human gastric carcinoma cell line SGC-7901 in vitro and in vivo. | |
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MedLine Citation:
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PMID: 19370777 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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AIM: To investigate the inhibitory effect of acetylshikonin on human gastric carcinoma cell line SGC-7901 and its mechanism. METHODS: MTT assay was used to assess the inhibitory effect of acetylshikonin on proliferation of SGC-7901 cells. Apoptosis-inducing effect was determined by flow cytometry and terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end-labeling with Hoechst staining. Expression of mRNA and protein in Bcl-2 and Bax was analyzed by reverse transcription-polymerase chain reaction and Western blot. Antitumor effect of acetylshikonin on a mouse SGC-7901 model was also determined. RESULTS: Forty-eight hours after treatment with acetylshikonin, MTT assay showed that acetylshikonin inhibited the proliferation of SGC-7901 cells in a dose-dependent manner. The half maximal inhibitory concentration of acetylshikonin to SGC-7901 cells was 0.428 +/- 0.07 mg/L. Cell shrinkage, nuclear pyknosis and chromatin condensation, which are the characteristics of cell apoptosis, were observed in treated SGC-7901 cells and the percentage of apoptosis increased in a dose-dependent manner. Acetylshikonin down-regulated the expression of Bcl-2 and up-regulated the expression of Bax in the treated SGC-7901 cells compared with the controls. The experiment in vivo showed that 0.5, 1, and 2 mg/kg of acetylshikonin significantly inhibited the growth of tumor in the mouse SGC-7901 model, with an inhibitory rate of 25.00%-55.76%. CONCLUSION: Acetylshikonin inhibits the growth of SGC-7901 cells in vitro and in vivo by inducing cell apoptosis. |
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Authors:
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Yun Zeng; Gang Liu; Li-Ming Zhou |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: World journal of gastroenterology : WJG Volume: 15 ISSN: 1007-9327 ISO Abbreviation: World J. Gastroenterol. Publication Date: 2009 Apr |
Date Detail:
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Created Date: 2009-04-16 Completed Date: 2009-05-26 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 100883448 Medline TA: World J Gastroenterol Country: China |
Other Details:
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Languages: eng Pagination: 1816-20 Citation Subset: IM |
Affiliation:
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Department of Pharmacology, West China Medical Center of Preclinical and Forensic Medicine, Sichuan University, Chengdu, Sichuan Province, China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Anthraquinones / pharmacology* Caspase 3 / metabolism Cell Cycle / drug effects Cell Line, Tumor / drug effects*, metabolism Cell Proliferation / drug effects Drugs, Chinese Herbal / pharmacology* Humans Male Mice Mice, Nude Neoplasm Transplantation Proto-Oncogene Proteins c-bcl-2 / genetics, metabolism Stomach Neoplasms* / metabolism, pathology bcl-2-Associated X Protein / genetics, metabolism |
| Chemical | |
Reg. No./Substance:
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0/Anthraquinones; 0/Drugs, Chinese Herbal; 0/Proto-Oncogene Proteins c-bcl-2; 0/bcl-2-Associated X Protein; 54984-93-9/acetylshikonin; EC 3.4.22.-/Caspase 3 |
| Comments/Corrections | |
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