Document Detail

Inhibitory effect of PGF-2 alpha on hCG-stimulated progesterone production in vitro by luteal cells from guinea-pigs at different stages of the oestrous cycle.
MedLine Citation:
PMID:  6956731     Owner:  NLM     Status:  MEDLINE    
Suspensions of luteal cells were prepared by collagenase dispersion of guinea-pig corpora lutea obtained at specific times during the oestrous cycle. Luteal cells incubated with hCG produced increased amounts of progesterone. For Days 3--13 of the oestrous cycle, the concentrations of hCG required for 50% of the maximum response were within the range, 1 x 10(-3) to 7 x 10(-3) i.u./ml, showing no marked loss of sensitivity to hCG with increasing luteal age. PGF-2 alpha (1 mumol/l), had no effect on basal production of progesterone but significantly inhibited hCG-stimulated progesterone production by luteal cells isolated on Days 7, 9, 10, 12 and 13 of the cycle. This concentration of PGF-2 alpha had no significant effect on progesterone production by luteal cells prepared earlier in the cycle (Days 3 and 5). It is concluded that (a) the luteolytic action of PGF-2 alpha in the guinea-pig is mediated, at least in part, by direct action on luteal cells, and (b) the cells from newly formed corpora lutea are resistant to the direct inhibitory action of PGF-2 alpha.
M C Richardson; M J Peddie
Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  Journal of reproduction and fertility     Volume:  66     ISSN:  0022-4251     ISO Abbreviation:  J. Reprod. Fertil.     Publication Date:  1982 Sep 
Date Detail:
Created Date:  1982-12-02     Completed Date:  1982-12-02     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0376367     Medline TA:  J Reprod Fertil     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  117-22     Citation Subset:  IM    
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MeSH Terms
Chorionic Gonadotropin / pharmacology
Corpus Luteum / cytology,  drug effects,  metabolism*
Guinea Pigs
Progesterone / biosynthesis*
Prostaglandins F / pharmacology*
Reg. No./Substance:
0/Chorionic Gonadotropin; 0/Prostaglandins F; 551-11-1/Dinoprost; 57-83-0/Progesterone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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