Document Detail

Inhibitory effect of NH4Cl on secretion of collagen in human gingival fibroblasts.
MedLine Citation:
PMID:  6593808     Owner:  NLM     Status:  MEDLINE    
The general protein synthesis in human gingival fibroblasts as measured by 14C-proline incorporation was only moderately inhibited by 10 mM NH4C1 during incubation for 36 h. The proportion secreted as noncollagen protein and recovered from the cellular fraction as collagen was not significantly affected, whereas a pronounced inhibitory effect on the secretion of collagen was evident after 24 h. This effect was dose dependent, with a significant inhibition of collagen secretion even at 2 mM ammonia. The applied concentrations of NH4C1 had no significant effect on the hydroxylation of prolyl residues in collagen. Ammonia had no inhibitory effect on the secretion of fibronectin, another major secretory protein from fibroblasts. When comparing different lysosomotropic agents; NH4C1, chloroquine and methylamine, the most prominent effect was consistently found to be an inhibition of the secretion of collagen.
K Helgeland
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Scandinavian journal of dental research     Volume:  92     ISSN:  0029-845X     ISO Abbreviation:  Scand J Dent Res     Publication Date:  1984 Oct 
Date Detail:
Created Date:  1984-12-12     Completed Date:  1984-12-12     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0270023     Medline TA:  Scand J Dent Res     Country:  DENMARK    
Other Details:
Languages:  eng     Pagination:  419-25     Citation Subset:  D; IM    
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MeSH Terms
Ammonium Chloride / pharmacology*
Cells, Cultured
Chloroquine / pharmacology
Collagen / antagonists & inhibitors,  secretion*
Dose-Response Relationship, Drug
Fibroblasts / drug effects
Fibronectins / secretion
Gingiva / drug effects*,  secretion
Methylamines / pharmacology
Time Factors
Reg. No./Substance:
0/Fibronectins; 0/Methylamines; 12125-02-9/Ammonium Chloride; 54-05-7/Chloroquine; 9007-34-5/Collagen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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