Document Detail

Inhibitory effect of Ginkgo biloba extract on fatty liver: Regulation of carnitine palmitoyltransferase 1a and fatty acid metabolism.
MedLine Citation:
PMID:  22988926     Owner:  NLM     Status:  In-Data-Review    
OBJECTIVE: To investigate the potential effect of Ginkgo biloba extract (GBE) on the prevention and treatment of nonalcoholic fatty liver disease (NAFLD).
METHODS: Male Wistar rats were divided into 4 groups (the control group, GBE group, high-fat diet [HFD] group and HFD + GBE group). The human hepatocellular carcinoma cell line (HepG2) was treated with GBE and its flavonoid ingredients. The fatty acid composition of the rat liver was analyzed with gas chromatography/time-of-flight mass spectrometry (GC/TOFMS). Triglyceride contents of both the rat liver and HepG2 cells were measured by enzymatic colorimetric method. The expressions of fatty acid metabolism-related genes were analyzed with real-time reverse transcription-polymerase chain reaction (RT-PCR). The protein expression and enzymatic activity were subsequently measured.
RESULTS: In rat livers, GBE reduced the elevations of hepatic triglyceride contents caused by HFD and the increased hepatic fatty acids were differentially affected by GBE. Notably, the expression and total activity of the fatty acid β-oxidation rate-limiting enzyme, carnitine palmitoyltransferase 1a (CPT1A), were also promoted with GBE ingestion. In HepG2 cells, GBE and its ingredients, quercetin, kaempferol and isorhamnetin, could decrease the cellular triglyceride content and upregulate the expression and total activity of CPT1A, respectively.
CONCLUSIONS: The triglyceride-lowering effect of GBE on the HFD rat liver is closely associated with the increased expression and activity of CPT1A, and the flavonoid ingredients are the major contributors of GBE.
Shi Dong Wang; Zuo Quan Xie; Jia Chen; Ke Wang; Ting Wei; Ai Hua Zhao; Qing Hua Zhang
Related Documents :
807706 - Oral absorption efficiency of acid-labile antibiotics from lipid-drug delivery systems.
18572606 - Tuning morphology and fluorescence of aggregated nanostructures of derived perylene dii...
19620386 - Responses of the hamster chorda tympani nerve to sucrose+acid and sucrose+citrate taste...
3042936 - Digestive and absorptive phase anomalies associated with the exocrine pancreatic insuff...
942206 - Rapid determination of dipicolinic acid in the spores of clostridium species by gas-liq...
8198056 - Plasma and tissue lipids of piglets fed formula containing saturated fatty acids from m...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of digestive diseases     Volume:  13     ISSN:  1751-2980     ISO Abbreviation:  J Dig Dis     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-09-19     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101302699     Medline TA:  J Dig Dis     Country:  Australia    
Other Details:
Languages:  eng     Pagination:  525-35     Citation Subset:  IM    
Copyright Information:
© 2012 The Authors. Journal of Digestive Diseases © 2012 Chinese Medical Association Shanghai Branch, Chinese Society of Gastroenterology, Renji Hospital Affiliated to Shanghai Jiaotong University School of Medicine and Wiley Publishing Asia Pty Ltd.
Center for Chinese Medical Therapy and Systems Biology, Shanghai University of Traditional Chinese Medicine, Shanghai, China; National Engineering Center for Biochip at Shanghai & Shanghai-MOST Key Laboratory of Health and Disease Genomics, Shanghai, China.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Factors influencing diagnosis of colorectal cancer: A hospital-based survey in China.
Next Document:  Role of nicotine in gallbladder carcinoma: A preliminary report.