Document Detail


Inhibitory action of omeprazole on acid formation in gastric glands and on H+,K+-ATPase isolated from human gastric mucosa.
MedLine Citation:
PMID:  3012768     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The inhibitory effect of omeprazole on acid formation has been studied in vitro in gastric glands and partly purified H+,K+-ATPase, prepared from mucosa obtained either from healthy subjects by gastroscopic biopsy or from gastric ulcer patients during antrectomy. The effect of omeprazole was compared with the inhibitory pattern of the H2-antagonist cimetidine. Acid production in the glands was determined by measuring the accumulation of 14C-aminopyrine. In glands isolated from patients, omeprazole inhibited acid production maximally stimulated by histamine, db-cAMP, and potassium in a dose-dependent manner, with an IC50 value of about 50 nM irrespective of the agonist used. In contrast, cimetidine inhibited only histamine-induced aminopyrine accumulation, with an IC50 of about 30 micron. The inhibitory effect of omeprazole in db-cAMP-stimulated glands from healthy volunteers was of the same magnitude as seen in glands from gastric ulcer patients. Basal aminopyrine accumulation in glands from both patients and healthy volunteers was almost totally inhibited by omeprazole, whereas cimetidine was without effect. Omeprazole also concentration-dependently inhibited the H+,K+-ATPase activity in isolated gastric membrane vesicles. The estimated IC50 value was 4 micron.
Authors:
B Elander; E Fellenius; R Leth; L Olbe; B Wallmark
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Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  Scandinavian journal of gastroenterology     Volume:  21     ISSN:  0036-5521     ISO Abbreviation:  Scand. J. Gastroenterol.     Publication Date:  1986 Apr 
Date Detail:
Created Date:  1986-07-02     Completed Date:  1986-07-02     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0060105     Medline TA:  Scand J Gastroenterol     Country:  NORWAY    
Other Details:
Languages:  eng     Pagination:  268-72     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adenosine Triphosphatases / antagonists & inhibitors*
Aminopyrine / metabolism
Anti-Ulcer Agents / pharmacology*
Benzimidazoles / pharmacology*
Cimetidine / pharmacology
Gastric Acid / metabolism*
Gastric Mucosa / drug effects*,  enzymology,  metabolism
H(+)-K(+)-Exchanging ATPase
Humans
Omeprazole
Stomach Ulcer / metabolism
Chemical
Reg. No./Substance:
0/Anti-Ulcer Agents; 0/Benzimidazoles; 51481-61-9/Cimetidine; 58-15-1/Aminopyrine; 73590-58-6/Omeprazole; EC 3.6.1.-/Adenosine Triphosphatases; EC 3.6.1.10/H(+)-K(+)-Exchanging ATPase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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