Document Detail

Inhibitory action of ganglioside GM3 on murine neuroblastoma cell proliferation: modulating effect of fetal calf serum.
MedLine Citation:
PMID:  7645939     Owner:  NLM     Status:  MEDLINE    
Exogenous gangliosides have been shown to exert a regulatory influence on the proliferation and differentiation of several cell lines in tissue culture. The effect of ganglioside GM3 on C-1300 murine neuroblastoma (MNB) cell proliferation and the modulating action of fetal calf serum (FCS) concentration in the culture media have been investigated. MNB cells were cultured in DMEM containing 1, 2.5, 5 or 10% FCS, and incubated with GM3 at concentrations ranging from 1.95 to 500 microM. Cell proliferation was assayed 4 days after the addition of GM3 using the CellTiter 96 Non-Radioactive Cell Proliferation Assay. GM3 inhibited MNB cell proliferation in a dose-dependent manner regardless of the FCS concentration in the culture media. However, the magnitude of this inhibitory effect was inversely proportional to the FCS concentration in the culture media. The addition of albumin to MNB cells cultured in DMEM containing 1% FCS exerted no effect on the antiproliferative action of GM3. FACS cell cycle analyses demonstrated that MNB cultured in DMEM containing 1% FCS had a higher proportion of cells in the G0/G1 compartment when compared to those cultured in 10% FCS. The enhanced response of MNB cells to GM3 observed in 1% FCS, may be due to a preferential action on cells in the G0/G1 stage of the cell cycle. These studies have demonstrated that the ganglioside GM3 inhibited MNB cell proliferation in tissue culture and this effect was modulated by FCS concentration in the culture media. Since protein-binding of GM3 by FCS does not appear to be the primary mechanism by which FCS exerts its antagonistic effects, we hypothesize that this may be due to the opposing action of stimulatory growth factors present in FCS.
C Zhang; A S Paller; B L Mirkin
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Anticancer research     Volume:  15     ISSN:  0250-7005     ISO Abbreviation:  Anticancer Res.     Publication Date:    1995 May-Jun
Date Detail:
Created Date:  1995-09-20     Completed Date:  1995-09-20     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8102988     Medline TA:  Anticancer Res     Country:  GREECE    
Other Details:
Languages:  eng     Pagination:  661-6     Citation Subset:  IM    
Children's Memorial Institute for Education and Research (CMIER), Children's Memorial Hospital, Department of Pediatrics and Pharmacology, Northwestern University Medical School, Chicago, IL 60614, USA.
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MeSH Terms
Cell Differentiation / drug effects
Cell Division / drug effects*
Culture Media
Culture Media, Serum-Free
Dose-Response Relationship, Drug
G(M3) Ganglioside / pharmacology*
Serum Albumin / pharmacology
Time Factors
Tumor Cells, Cultured
Grant Support
Reg. No./Substance:
0/Culture Media; 0/Culture Media, Serum-Free; 0/G(M3) Ganglioside; 0/Serum Albumin

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