| Inhibitory effects of antioxidant reagent in reactive oxygen species generation and penetration of appressoria of Alternaria alternata Japanese pear pathotype. | |
| | |
MedLine Citation:
|
PMID: 20701480 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
In the Japanese pear pathotype of Alternaria alternata, H2O2 is generated solely from penetration pegs and not from other portions of subcuticular hyphae within the pectin layers of host leaves. A close association between H2O2 generation and fungal aggressiveness is expected because the pegs are important for fungal penetration into the host epidermis. To determine the potential role of reactive oxygen species in microbial pathogenicity, we studied the inhibitory effects of the antioxidant reagent ascorbic acid and the NADPH oxidase inhibitor diphenylene iodonium on infection of the pathogen. In our study, we showed H2O2 generation to be inhibited by inoculation with the mixture of ascorbic acid or diphenylene iodonium and spores at the pegs in the spore-inoculated host leaves. The decrease of generation in the pegs resulted in penetration failure, indicating that H2O2 generation probably contributed to strengthening of the penetration and probably was recruited by NADPH oxidase. |
| | |
Authors:
|
Gang-Su Hyon; Ken-Ichi Ikeda; Naoki Hosogi; Takeshi Shinogi; Pyoyun Park |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Phytopathology Volume: 100 ISSN: 0031-949X ISO Abbreviation: Phytopathology Publication Date: 2010 Sep |
Date Detail:
|
Created Date: 2010-08-12 Completed Date: 2010-10-19 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9427222 Medline TA: Phytopathology Country: United States |
Other Details:
|
Languages: eng Pagination: 840-7 Citation Subset: IM |
Affiliation:
|
Laboratory of Stress Cytology, Graduate School of Agricultural Science, Kobe University , Rokkodai, Nada, Kobe, Japan. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Alternaria
/
physiology*,
ultrastructure Antioxidants / pharmacology* Ascorbic Acid / pharmacology* Host-Parasite Interactions Hydrogen Peroxide / metabolism Hyphae / drug effects, physiology Onium Compounds / pharmacology Plant Diseases / microbiology* Plant Leaves / drug effects, microbiology Plant Proteins / antagonists & inhibitors, metabolism Pyrus / microbiology* Reactive Oxygen Species |
| Chemical | |
Reg. No./Substance:
|
0/Antioxidants; 0/Onium Compounds; 0/Plant Proteins; 0/Reactive Oxygen Species; 244-54-2/diphenyleneiodonium; 50-81-7/Ascorbic Acid; 7722-84-1/Hydrogen Peroxide |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: A Randomized Pilot Study of Acupuncture as an Adjunct Therapy in Adult Asthmatic Patients.
Next Document: Mass spectrometric analysis reveals remnants of host-pathogen molecular interactions at the starch g...