Document Detail


Inhibitory effects of antioxidant reagent in reactive oxygen species generation and penetration of appressoria of Alternaria alternata Japanese pear pathotype.
MedLine Citation:
PMID:  20701480     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In the Japanese pear pathotype of Alternaria alternata, H2O2 is generated solely from penetration pegs and not from other portions of subcuticular hyphae within the pectin layers of host leaves. A close association between H2O2 generation and fungal aggressiveness is expected because the pegs are important for fungal penetration into the host epidermis. To determine the potential role of reactive oxygen species in microbial pathogenicity, we studied the inhibitory effects of the antioxidant reagent ascorbic acid and the NADPH oxidase inhibitor diphenylene iodonium on infection of the pathogen. In our study, we showed H2O2 generation to be inhibited by inoculation with the mixture of ascorbic acid or diphenylene iodonium and spores at the pegs in the spore-inoculated host leaves. The decrease of generation in the pegs resulted in penetration failure, indicating that H2O2 generation probably contributed to strengthening of the penetration and probably was recruited by NADPH oxidase.
Authors:
Gang-Su Hyon; Ken-Ichi Ikeda; Naoki Hosogi; Takeshi Shinogi; Pyoyun Park
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Phytopathology     Volume:  100     ISSN:  0031-949X     ISO Abbreviation:  Phytopathology     Publication Date:  2010 Sep 
Date Detail:
Created Date:  2010-08-12     Completed Date:  2010-10-19     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9427222     Medline TA:  Phytopathology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  840-7     Citation Subset:  IM    
Affiliation:
Laboratory of Stress Cytology, Graduate School of Agricultural Science, Kobe University , Rokkodai, Nada, Kobe, Japan.
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MeSH Terms
Descriptor/Qualifier:
Alternaria / physiology*,  ultrastructure
Antioxidants / pharmacology*
Ascorbic Acid / pharmacology*
Host-Parasite Interactions
Hydrogen Peroxide / metabolism
Hyphae / drug effects,  physiology
Onium Compounds / pharmacology
Plant Diseases / microbiology*
Plant Leaves / drug effects,  microbiology
Plant Proteins / antagonists & inhibitors,  metabolism
Pyrus / microbiology*
Reactive Oxygen Species
Chemical
Reg. No./Substance:
0/Antioxidants; 0/Onium Compounds; 0/Plant Proteins; 0/Reactive Oxygen Species; 244-54-2/diphenyleneiodonium; 50-81-7/Ascorbic Acid; 7722-84-1/Hydrogen Peroxide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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