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Inhibitory Effect of p53 Upregulated Modulator of Apoptosis Targeting siRNA on Hypoxia/Reoxygenation-Induced Cardiomyocyte Apoptosis in Rats.
MedLine Citation:
PMID:  22760064     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Objectives: The effect of p53 upregulated modulator of apoptosis (PUMA) in hypoxia/reoxygenation-induced cardiomyocyte injuries in rats was investigated. Methods: PUMA-targeting (si-PUMA) and scramble siRNAs were designed and transfected into primarily rat cardiomyocytes in vitro. Results: RT-PCR and Western blot analysis showed that 50 nmol/l of si-PUMA can specifically inhibit PUMA expression. MTT assay and lactate dehydrogenase activity detection showed that the cell survival rate in the si-PUMA group was enhanced and that the lactate dehydrogenase enzymatic activity dramatically decreased compared with the control group (p < 0.01). Spectrophotometry, as well as annexin V and propidium iodide staining, combined with flow cytometry, revealed that caspase-3 activity in the si-PUMA group was downregulated and the apoptotic rate was decreased (p < 0.01). RT-PCR also showed that Bax expression was downregulated and Bcl-2 expression was upregulated in the si-PUMA group, compared with the control group (p < 0.05). si-PUMA protects cardiomyocytes from apoptosis. Conclusion: PUMA mediates hypoxia/reoxygenation-induced cardiomyocyte apoptosis, which can be a potential target of gene therapy for ischemia/reperfusion cardiomyocyte injuries.
Authors:
Shuo Tu; Zhuo-Qi Liu; Jing-Jing Fu; Wei-Feng Zhu; Da-Ya Luo; Fu-Sheng Wan
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-6-30
Journal Detail:
Title:  Cardiology     Volume:  122     ISSN:  1421-9751     ISO Abbreviation:  -     Publication Date:  2012 Jun 
Date Detail:
Created Date:  2012-7-4     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  1266406     Medline TA:  Cardiology     Country:  -    
Other Details:
Languages:  ENG     Pagination:  93-100     Citation Subset:  -    
Copyright Information:
Copyright © 2012 S. Karger AG, Basel.
Affiliation:
Department of Biochemistry and Molecular Biology, Basic Medical College of Nanchang University, Nanchang, China.
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