Document Detail


Inhibitory effect of carotenoids on the degranulation of mast cells via suppression of antigen-induced aggregation of high affinity IgE receptors.
MedLine Citation:
PMID:  19700409     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Carotenoids have been demonstrated to possess antioxidative and anti-inflammatory effects. However, there is no report that the effects of carotenoids on degranulation of mast cell is critical for type I allergy. In this study, we focused on the effect of carotenoids on antigen-induced degranulation of mast cells. Fucoxanthin, astaxanthin, zeaxanthin, and beta-carotene significantly inhibited the antigen-induced release of beta-hexosaminidase in rat basophilic leukemia 2H3 cells and mouse bone marrow-derived mast cells. Those carotenoids also inhibited antigen-induced aggregation of the high affinity IgE receptor (Fc epsilonRI), which is the most upstream of the degranulating signals of mast cells. Furthermore, carotenoids inhibited Fc epsilonRI-mediated intracellular signaling, such as phosphorylation of Lyn kinase and Fyn kinase. It suggests that the inhibitory effect of carotenoids on the degranulation of mast cells were mainly due to suppressing the aggregation of Fc epsilonRI followed by intracellular signaling. In addition, those carotenoids inhibited antigen-induced translocation of Fc epsilonRI to lipid rafts, which are known as platforms of the aggregation of Fc epsilonRI. We assume that carotenoids may modulate the function of lipid rafts and inhibit the translocation of Fc epsilonRI to lipid rafts. This is the first report that focused on the aggregation of Fc epsilonRI to investigate the mechanism of the inhibitory effects on the degranulation of mast cells and evaluated the functional activity of carotenoids associated with lipid rafts.
Authors:
Shota Sakai; Tatsuya Sugawara; Kiminori Matsubara; Takashi Hirata
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-08-21
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  284     ISSN:  1083-351X     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  2009 Oct 
Date Detail:
Created Date:  2009-10-05     Completed Date:  2009-11-10     Revised Date:  2013-05-31    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  28172-9     Citation Subset:  IM    
Affiliation:
Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University, Kyoto 606-8502, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antigens / immunology*
Carotenoids / immunology,  pharmacology*
Cell Degranulation / drug effects*,  immunology
Cell Line
Mast Cells* / drug effects,  immunology
Membrane Microdomains / metabolism
Mice
Mice, Inbred BALB C
Monoterpenes / pharmacology
Rats
Receptor Aggregation
Receptors, IgE / chemistry,  immunology*
Signal Transduction / physiology
beta-N-Acetylhexosaminidases / metabolism
Chemical
Reg. No./Substance:
0/Antigens; 0/Monoterpenes; 0/Receptors, IgE; 36-88-4/Carotenoids; EC 3.2.1.52/beta-N-Acetylhexosaminidases
Comments/Corrections

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