Document Detail

Inhibitory Activity of Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum (Higher Basidiomycetes) on Transformed Cells by Human Papillomavirus.
MedLine Citation:
PMID:  24941039     Owner:  NLM     Status:  In-Data-Review    
In this study, we investigated the effects of the aqueous extracts of Lingzhi or Reishi medicinal mushroom, Ganoderma lucidum, obtained from three localities (China; and Morelos and Michoacan, Mexico) on cervical cells transformed by human papillomavirus (HeLa and SiHa) and C-33A cancer cells. The cells were plated in DMEM medium supplemented, and were incubated in the presence of different concentrations of G. lucidum for 24 h. Cell proliferation was determined by MTT colorimetric assay and viability by trypan blue assay. Inhibitory dose was determined (IC50) of the three different extracts of G. lucidum in the culture cell lines mentioned above. The apoptosis process was confirmed by nuclear DNA fragmentation and the cell cycle was determined by flow cytometry. The results showed that aqueous extracts G. lucidum obtained from three localities produced inhibition in the proliferation of VPH transformed cells; they also induced apoptosis and cell cycle arrest in HeLa, SiHa, and C-33A cancer cells. Therefore, it was found that aqueous extracts G. lucidum obtained from three different locations produced inhibitory effect on cancer cells and may have a potential therapeutic use for the prevention and treatment of this disease.
Eva Hernandez-Marquez; Alfredo Lagunas-Martinez; Victor H Bermudez-Morales; Ana I Burgete-Garcfa; Ismael Leon-Rivera; Elizur Montiel-Arcos; Enrique Garcia-Villa; Patricio Gariglio; Vicente Madrid-Marina V; Raul N Ondarza-Vidaurreta
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  International journal of medicinal mushrooms     Volume:  16     ISSN:  1940-4344     ISO Abbreviation:  Int J Med Mushrooms     Publication Date:  2014  
Date Detail:
Created Date:  2014-06-19     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100886202     Medline TA:  Int J Med Mushrooms     Country:  United States    
Other Details:
Languages:  eng     Pagination:  179-87     Citation Subset:  IM    
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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