Document Detail

Inhibitors of protein synthesis in eukarytic cells. Comparative effects of some amaryllidaceae alkaloids.
MedLine Citation:
PMID:  944052     Owner:  NLM     Status:  MEDLINE    
The effects of eighteen compounds obtained from bulbs of the Amaryllidaceae family were tested on (a) animal cell growth, (b) DNA, RNA and protein synthesis by intact cells and (c) protein synthesis in cell-free systems. Dihydrolycorine, haemanthamine, lycorine, narciclasine, pretazettine and pseudolycorine halted HeLa cell growth at 10(-1) mM or lower concentrations. These compounds at their growth inhibitory concentrations block protein synthesis in ascites cells and stabilize HeLa cell polysomes in vivo. Endomyocarditis virus RNA-directed cell-free polypeptide synthesis by an ascites S-30 extract and acetyl-[14C]leucyl-puromycin formation by ascites ribosomes are also inhibited by the six compounds indicated above. It is therefore concluded that they halt protein synthesis in eukaryotic cells by inhibiting the peptide bone formation step.
A Jimenez; A Santos; G Alonso; D Vazquez
Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  425     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  1976 Mar 
Date Detail:
Created Date:  1976-07-06     Completed Date:  1976-07-06     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  342-8     Citation Subset:  IM    
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MeSH Terms
Alkaloids / pharmacology*
Carcinoma, Krebs 2 / metabolism
Hela Cells / drug effects,  metabolism
Plant Extracts / pharmacology*
Protein Biosynthesis / drug effects*
Species Specificity
Structure-Activity Relationship
Reg. No./Substance:
0/Alkaloids; 0/Plant Extracts

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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