Document Detail


Inhibitors of phosphatidylinositol 3-kinase activity prevent cell cycle progression and induce apoptosis at the M/G1 transition in CHO cells.
MedLine Citation:
PMID:  16389452     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Phosphatidylinositol 3-kinase (PI3-kinase) activity has been implicated in regulating cell cycle progression at distinct points in the cell cycle by preventing cell cycle arrest or apoptosis. In this study, the role of PI3-kinase activity during the entire G1 phase of the ongoing cell cycle was studied in Chinese hamster ovary (CHO) cells synchronized by mitotic shake-off. We show that inhibition of PI3-kinase activity during and 2 h after mitosis inhibited cell cycle progression into S phase. In the presence of the PI3-kinase inhibitor wortmannin or LY294002, cells were arrested during early G1 phase, leading to the expression of the cleaved caspase-3, a central mediator of apoptosis. These results demonstrate that PI3-kinase activity is required for progression through the M/G1 phase. In the absence of PI3-kinase activity, cells are induced for apoptosis in this particular phase of the cell cycle.
Authors:
A van Opstal; J Boonstra
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Cellular and molecular life sciences : CMLS     Volume:  63     ISSN:  1420-682X     ISO Abbreviation:  Cell. Mol. Life Sci.     Publication Date:  2006 Jan 
Date Detail:
Created Date:  2006-01-17     Completed Date:  2006-03-16     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9705402     Medline TA:  Cell Mol Life Sci     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  220-8     Citation Subset:  IM    
Affiliation:
Cellular Architecture & Dynamics, Utrecht University, Padualaan 8, 3584 CH, Utrecht, The Netherlands.
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MeSH Terms
Descriptor/Qualifier:
1-Phosphatidylinositol 3-Kinase / physiology*
Animals
Apoptosis / drug effects*
CHO Cells
Cell Cycle / drug effects*
Cricetinae
Cricetulus
G1 Phase / drug effects*
Microfilaments / physiology
Mitosis
Protein Kinase Inhibitors / pharmacology*
Chemical
Reg. No./Substance:
0/Protein Kinase Inhibitors; EC 2.7.1.137/1-Phosphatidylinositol 3-Kinase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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