Document Detail


Inhibitor studies of dissimilative Fe(III) reduction by Pseudomonas sp. strain 200 ("Pseudomonas ferrireductans")
MedLine Citation:
PMID:  2428308     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Aerobic respiration and dissimilative iron reduction were studied in pure, batch cultures of Pseudomonas sp. strain 200 ("Pseudomonas ferrireductans"). Specific respiratory inhibitors were used to identify elements of electron transport chains involved in the reduction of molecular oxygen and Fe(III). When cells were grown at a high oxygen concentration, dissimilative iron reduction occurred via an abbreviated electron transport chain. The induction of alternative respiratory pathways resulted from growth at low oxygen tension (less than 0.01 atm [1 atm = 101.29 kPa]). Induced cells were capable of O2 utilization at moderately increased rates; dissimilative iron reduction was accelerated by a factor of 6 to 8. In cells grown at low oxygen tension, dissimilative iron reduction appeared to be uncoupled from oxidative phosphorylation. Models of induced and uninduced electron transport chains, including a mathematical treatment of chemical inhibition within the uninduced, aerobic electron transport system, are presented. In uninduced cells respiring anaerobically, electron transport was limited by ferrireductase activity. This limitation may disappear among induced cells.
Authors:
R G Arnold; T J DiChristina; M R Hoffmann
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  52     ISSN:  0099-2240     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  1986 Aug 
Date Detail:
Created Date:  1986-10-23     Completed Date:  1986-10-23     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  281-9     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
2,4-Dinitrophenol
Aerobiosis
Azides / pharmacology
Chloramphenicol / pharmacology
Dicumarol / pharmacology
Dicyclohexylcarbodiimide / pharmacology
Dinitrophenols / pharmacology
Electron Transport / drug effects
Hydroxyquinolines / pharmacology
Iron / metabolism*
Kinetics
Oxidation-Reduction
Oxygen Consumption / drug effects*
Pseudomonas / drug effects,  growth & development,  metabolism*
Quinacrine / pharmacology
Rotenone / pharmacology
Sodium Azide
Sodium Cyanide / pharmacology
Chemical
Reg. No./Substance:
0/Azides; 0/Dinitrophenols; 0/Hydroxyquinolines; 143-33-9/Sodium Cyanide; 26628-22-8/Sodium Azide; 341-88-8/2-(n-heptyl)-4-hydroxyquinoline N-oxide; 51-28-5/2,4-Dinitrophenol; 538-75-0/Dicyclohexylcarbodiimide; 56-75-7/Chloramphenicol; 66-76-2/Dicumarol; 7439-89-6/Iron; 83-79-4/Rotenone; 83-89-6/Quinacrine
Comments/Corrections
Erratum In:
Appl Environ Microbiol 1986 Oct;52(4):974

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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