| Inhibitor of differentiation 1 (ID1) promotes cell survival and proliferation of prostate epithelial cells. | |
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MedLine Citation:
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PMID: 20186495 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Id1 (inhibitor of differentiation 1) is a member of the bHLH protein family. Consistent with its role in promoting proliferation and inhibiting differentiation, Id1 expression is low or negligible in normal prostate epithelial cells but is high in prostate cancer. Ectopic expression of Id1 in normal prostate epithelial cells could therefore provide a model for understanding early events involved in initiation of prostate cancer. Over-expression of Id1 immortalized but did not transform ventral prostate epithelial cells (Id1-RPE). Immortalization was associated with decreased Cdkn2a, Cdkn1a, androgen receptor and increased Tert expression. Gene expression profiling over successive doublings was used to identify transcriptomic changes involved during immortalization (Tieg, Jun, alpha actin, Klf10, Id2) and in maintaining the immortalized phenotype (Igfbp3, Igfbp5, Mmp2, Tgfb3). Network analysis indicated that Id1 promotes cancer/tumor morphology, cell cycle and epithelial to mesenchymal transition by influencing AP1, tnf, tgfbeta, PdgfBB and estradiol pathways. During immortalization, the expression of majority of differentially expressed genes reduced over progressive doublings suggesting a decline in transcriptional regulatory mechanisms. The associated molecular/gene expression profile of Id1-RPE cells provides an opportunity to understand the molecular pathways associated with prostate epithelial cell survival and proliferation. |
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Authors:
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Michelle Schmidt; Ananthi J Asirvatham; Jaideep Chaudhary |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2010-02-25 |
Journal Detail:
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Title: Cellular & molecular biology letters Volume: 15 ISSN: 1689-1392 ISO Abbreviation: Cell. Mol. Biol. Lett. Publication Date: 2010 Jun |
Date Detail:
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Created Date: 2010-03-25 Completed Date: 2010-07-05 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9607427 Medline TA: Cell Mol Biol Lett Country: Poland |
Other Details:
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Languages: eng Pagination: 272-95 Citation Subset: IM |
Affiliation:
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Washington State University, Spokane, 99164, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Agar Androgens / pharmacology Animals Biological Markers / metabolism Cell Line, Transformed Cell Proliferation / drug effects Cell Survival / drug effects Colony-Forming Units Assay Computational Biology Cyclin-Dependent Kinase Inhibitor p16 / metabolism Cyclin-Dependent Kinase Inhibitor p21 / metabolism Epithelial Cells / cytology*, drug effects, metabolism* Gene Expression Profiling Gene Expression Regulation / drug effects Gene Regulatory Networks Humans Inhibitor of Differentiation Protein 1 / metabolism* Karyotyping Male Mice Oligonucleotide Array Sequence Analysis Prostate / cytology* Rats Rats, Sprague-Dawley Receptors, Androgen / genetics, metabolism Telomerase / metabolism |
| Grant Support | |
ID/Acronym/Agency:
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G12RR03062/RR/NCRR NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Androgens; 0/Biological Markers; 0/Cyclin-Dependent Kinase Inhibitor p16; 0/Cyclin-Dependent Kinase Inhibitor p21; 0/ID1 protein, human; 0/Inhibitor of Differentiation Protein 1; 0/Receptors, Androgen; 9002-18-0/Agar; EC 2.7.7.49/Telomerase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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