|Inhibition of uncoupling protein 2 with genipin exacerbates palmitate-induced hepatic steatosis.|
|Jump to Full Text|
|PMID: 23151243 Owner: NLM Status: MEDLINE|
|BACKGROUND: Uncoupling protein 2 (UCP2) was reported to be involved in lipid metabolism through regulating the production of superoxide anion. However, the role of UCP2 in hepatocytes steatosis has not been determined. We hypothesized that UCP2 might regulate hepatic steatosis via suppressing oxidative stress.
RESULTS: We tested this hypothesis in an in vitro model of hepatocytic steatosis in HepG2 cell lines induced by palmitic acid (PA). We found that treatment with PA induced an obvious lipid accumulation in HepG2 cells and a significant increase in intracellular triglyceride content. Moreover, the specific inhibition of UCP2 by genipin remarkably exacerbated PA-induced hepatocytes steatosis. Interestingly, the PA-induced superoxide overproduction can also be enhanced by incubation with genipin. In addition, administration with the antioxidant tempol abolished genipin-induced increase in intracellular lipid deposition. We further found that genipin significantly increased the protein expression of fatty acid translocase (FAT)/CD36.
CONCLUSIONS: These findings suggest that UCP2 plays a protective role in PA-induced hepatocytic steatosis through ameliorating oxidative stress.
|Shuangtao Ma; Dachun Yang; De Li; Yan Tan; Bing Tang; Yongjian Yang|
Related Documents :
|23582513 - Low docosahexaenoic acid status is associated with reduced indices in cortical integrit...
22983303 - On the role of the mitochondrial 2-oxoglutarate dehydrogenase complex in amino acid met...
7572913 - Control of gastric acid with high dose h2-receptor antagonists after omeprazole failure...
|Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2012-11-14|
|Title: Lipids in health and disease Volume: 11 ISSN: 1476-511X ISO Abbreviation: Lipids Health Dis Publication Date: 2012|
|Created Date: 2012-12-21 Completed Date: 2013-03-04 Revised Date: 2013-07-11|
Medline Journal Info:
|Nlm Unique ID: 101147696 Medline TA: Lipids Health Dis Country: England|
|Languages: eng Pagination: 154 Citation Subset: IM|
|Department of Cardiology, General Hospital of PLA Chengdu Military Area Command, 270 Rongdu Rd, Tianhui Town, Jinniu District, Chengdu 610083, Sichuan Province, People's Republic of China.|
|APA/MLA Format Download EndNote Download BibTex|
Fatty Liver / chemically induced*, metabolism, pathology
Hep G2 Cells
Ion Channels / antagonists & inhibitors*
Iridoids / pharmacology*
Lipid Metabolism / drug effects
Mitochondrial Proteins / antagonists & inhibitors*
Oxidative Stress / drug effects
Palmitic Acid / toxicity*
Triglycerides / metabolism
|0/Antigens, CD36; 0/Ion Channels; 0/Iridoids; 0/Mitochondrial Proteins; 0/Triglycerides; 0/mitochondrial uncoupling protein 2; 57-10-3/Palmitic Acid; 6902-77-8/genipin|
Journal ID (nlm-ta): Lipids Health Dis
Journal ID (iso-abbrev): Lipids Health Dis
Publisher: BioMed Central
Copyright ©2012 Ma et al.; licensee BioMed Central Ltd.
Received Day: 10 Month: 5 Year: 2012
Accepted Day: 6 Month: 11 Year: 2012
collection publication date: Year: 2012
Electronic publication date: Day: 14 Month: 11 Year: 2012
Volume: 11First Page: 154 Last Page: 154
PubMed Id: 23151243
Publisher Id: 1476-511X-11-154
|Inhibition of uncoupling protein 2 with genipin exacerbates palmitate-induced hepatic steatosis|
|Shuangtao Ma1||Email: firstname.lastname@example.org|
|Dachun Yang1||Email: email@example.com|
|De Li1||Email: firstname.lastname@example.org|
|Yan Tan1||Email: email@example.com|
|Bing Tang1||Email: firstname.lastname@example.org|
|Yongjian Yang1||Email: email@example.com|
1Department of Cardiology, General Hospital of PLA Chengdu Military Area Command, 270 Rongdu Rd., Tianhui Town, Jinniu District, Chengdu, 610083, Sichuan Province, People’s Republic of China
Hepatic steatosis is an important process in the development of nonalcoholic fatty liver disease that is a major component of metabolic syndrome and a significant risk factor of cardiovascular disease . The accumulation of excess neutral fat within hepatocytes is closely related to obesity and overweight , suggesting hepatic steatosis mostly results from metabolic disturbance of lipids . It was reported that increased level of free fatty acid (FA) is associated with the onset of fatty liver disease and hepatic steatosis . However, the molecular mechanism underlying FA-induced hepatic fat deposition remains to be determined.
Uncoupling protein 2 (UCP2) belongs to the mitochondrial carrier family and has been thought to be a metabolic sensor coupling excess FA to lipotoxity . Not only the plasma FA levels are accompanied by the increased UCP2 expression but also the FA can stimulate the activity of the promoter regions of UCP2 gene . Moreover, the UCP2 is suggested to promote mitochondrial FA oxidation while limiting mitochondrial catabolism of pyruvate . These findings indicate that UCP2 might be involved in the development of FA-induced hepatic steatosis.
Attenuation of hepatic oxidative stress inhibits fat deposition, indicating that reactive oxygen species (ROS) might play a central role in the development of hepatic steatosis . UCP2 has been linked to mitochondria-derived ROS production. The previous studies have demonstrated that UCP2 ablation can enhance the oxidative stress though decreasing the proton leak . In addition, ROS is generated during the metabolism of free FA in mitochondria . Thus, UCP2 might be involved in hepatic steatosis via regulating ROS production in the process of excess FA oxidation. As UCP2 interacts to the FA translocase (FAT)/CD36, the uptake of FA might also be regulated by UCP2 .
Therefore, we hypothesized that UCP2 might regulate hepatic steatosis though a ROS-dependent pathway. In the present study, we present a cellular model of hepatic steatosis induced by palmitic acid (PA). We report that inhibition of UCP2 by genipin increases ROS production and enhances hepatocytic lipid deposition induced by PA. We also found that genipin-mediated lipid deposition is linked to the upregulation of FAT/CD36.
Intracellular lipid accumulation was examined using Oil Red O staining. Treating cultured hepatocytes with PA (250 μmol/L) medium for 24 h resulted in steatosis; in contrast, little lipid droplet was found in the untreated cells (Figure 1A). When cultured hepatocytes were incubated with PA plus UCP2 inhibitor genipin (5 μmol/L), the cellular lipid inclusions were significantly increased compared with cells treated with PA alone (Figure 1A). Intracellular triglyceride level was significantly increased from control by treatment for 24 h with PA (p < 0.01, Figure 1B) and was further elevated by incubating with genipin (p < 0.01, Figure 1B).
Superoxide anion production in hepatocyte was assessed by dihydroethidium (DHE) staining. We found that DHE fluorescence was significantly higher in PA-treated cells than untreated ones (Figure 2A, B and D). Additionally, DHE fluorescence in hepatocyte treated with PA plus genipin was markedly higher than PA-treated cells (Figure 2B, C and D).
To confirm the central role of oxidative stress in UCP2 inhibition-mediated enhancement of lipid deposition in hepatocytes, the antioxidant tempol was applied. We found that blocking oxidative stress with tempol reversed genipin-induced further increase in intracellular lipid deposition (p < 0.05, Figure 3A, B, C and D).
Treatment with PA significantly increased the protein expressions of UCP2 and FAT/CD36 in cultured hepatocytes (p < 0.01, Figure 4A and B). Surprisingly, inhibition of UCP2-mediated proton leak with genipin further increased the expression levels of UCP2 and FAT/CD36 (p < 0.05 or p < 0.01, Figure 4A and B).
In this study, we assessed the role of UCP2 in regulating ROS production in cultured hepatocytes under the condition of FA treatment and established a critical role of UCP2 in the pathogenesis of hepatic steatosis. We showed that nutrient excess in the form of high PA medium resulted in a rapid development of hepatic steatosis that was enhanced by inhibiting UCP2 with genipin. We also demonstrated that PA caused production of superoxide was enhanced by genipin, while antioxidant tempol blocked genipin-mediated steatosis. In addition, we found that protein expressions of UCP2 and FAT/CD36 were significantly upregulated by both PA and genipin.
The UCP2 protein expression has been linked to the occurrence of nonalcoholic fatty liver disease . The expression level of UCP2 in normal hepatocyte is very low but can be significantly upregulated under the obese condition . Several studies have demonstrated that hepatic steatosis is associated with increased expression of UCP2 expression . However, the exact role of UCP2 in the development of hepatic lipid deposition has not been determined. The present study shows that inhibition of UCP2 accelerates hepatic steatosis, indicating that UCP2 might play a compensatory protective role in PA-induced hepatic fat deposition. This finding suggests that the drugs upregulating UCP2 might become a novel strategy for fighting nonalcoholic fatty liver disease.
The current concept suggests that the oxidative stress plays an important role in the development of hepatic steatosis. UCP2, a well-established uncoupling protein, has been viewed as an inhibitor of mitochondrial ROS production . The present study demonstrates that the tempol, a well-known antioxidant, blocks UCP2 inhibition mediated hepatic steatosis. This finding suggests that UCP2 inhibition-caused liver fat deposition is dependent on the overproduction of superoxide.
UCP2 has been implicated in physiological and pathological processes related to lipid metabolism, especially in the uptake and oxidation of free FA [16,17]. The enzymes responsible for FA metabolism, such as peroxisome proliferator-activated receptor α and FAT/CD36, were linked to UCP2 . The present study found that UCP2 inhibition-associated lipid deposition is related to the upregulation of FAT/CD36, indicating the uptake of PA by hepatocyte is increased. The genipin-induced upregulation of UCP2 might be a compensatory effect.
In conclusion, the present results demonstrate that UCP2 plays a protective role in free FA-induced hepatic steatosis. Moreover, the UCP2-mediated lipid deposition is attributed to the production of superoxide. The present findings may contribute to the insights into the clinical prevention and management of nonalcoholic fatty liver disease.
HepG2 cells were obtained from the Shanghai Institute of Cell Biology (Shanghai, China). Cells were grown in Dulbecco’s modified eagle’s medium (DMEM; Invitrogen Co., Carlsbad, California, USA) with 10% FBS in the presence of 100 U/mL penicillin and 100 μg/mL streptomycin and maintained at 37°C in a humidified atmosphere containing 5% (v/v) CO2.
Cultured HepG2 cell were plated on cover slides in six-well plates and maintained in culture media until the cells reached 95% confluence. Experiment 1: The cells were incubated for 24 h with control medium, PA medium (addition of 250 μmol/L PA) and PA plus genipin (addition of 5 μmol/L genipin) supplemented with identical glucose and serum concentrations. This PA concentration was used because it has been successfully used in the previous studies. After that, the cells were washed with phosphate-buffered saline, fixed with 4% paraformaldehyde, and stained with Oil Red O, which detects neutral lipids such as cholesteryl esters and triglyceride . Intracellular triglyceride was detected. Experiment 2: The cells were divided into three groups: PA (treated with 250 μmol/L of PA), PA + genipin (treated with 250 μmol/L of OA plus 5 μmol/L of genipin) and PA + genipin + tempol (treated with 250 μmol/L of OA, 5 μmol/L of genipin and 1 mmol/L of tempol). After incubation for 24 h, Oil Red O staining of the cells was performed, and the intracellular triglyceride was measured. The cells were collected for the detection of UCP2 and FAT/CD36 expressions.
To assess superoxide production, the HepG2 cells were cultured and treated as above. The medium was removed, and the cells were rinsed twice with PBS. After that, the cells were incubated in the dark with dihydroethidium (DHE; Sigma-Aldrich, St Louis, MO) diluted in Krebs (40 μmol/l) for 30 min at 37°C followed by a 15 min wash in DHE-free Krebs. To quantitate the DHE fluorescence, the glass slides were placed under the Leica DM LB2 Fluorescent Microscope (Leica, Wetzlar, Germany) outfitted with a rhodamine filter set .
Cell lysate was prepared by lysing the cells with buffer containing 1% Triton X-100, 150 mM NaCl, 1 mM EDTA, 2.5 mM sodium pyrophosphate, 1 mM β-glycerophosphate, 1 mM Na3VO4, 1 μg/mL leupeptin, 1 μg/mL aprotinin, and 20 mM Tris (pH 7.5). Protein concentration was determined by Bio-Rad protein assay reagent (Bio-Rad Laboratories, USA). Lysate was electrophoretically transferred onto a nitrocellulose membrane and immunoblotted with rabbit anti-UCP2 and FAT/CD36 IgG (1:500 dilution, Sigma-Aldrich Co., USA). The blots were incubated with a horseradish peroxidase-conjugated secondary antibody (1:1000 dilution, Santa Cruz Biotechnology, USA), and the bound antibody was visualized using a colored reaction. The relative band intensity was quantified by the use of Quantity One software (Bio-Rad). Equal loading of protein was confirmed by measuring β-actin expression .
Data are presented as mean ± SEM. Comparisons between groups were determined by one-way ANOVA with a post hoc Student’s t-test (SPSS Inc., Chicago, IL). Probabilities of p < 0.05 were considered statistically significant.
FA: Fatty acid; FAT/CD36: Fatty acid translocase; PA: Palmitic acid; ROS: Reactive oxygen species; UCP2: Uncoupling protein 2; DHE: Dihydroethidium.
The authors declare that they have no competing interests.
SM carried out the Oil Red O staining and drafted the manuscript. DY carried out the cell studies. DL participated in the molecular studies. YT participated in the design of the study. BT performed the statistical analysis. YY conceived of the study, and participated in its design and coordination and helped to draft the manuscript. All authors read and approved the final manuscript.
This work was supported by the Natural Science Foundation of China (No. 81100232, to Shuangtao Ma) and the Research Foundation from General Hospital of PLA Chengdu Military Area Command (No. 2011YG-B22, to Shuangtao Ma).
|Williamson RM,Price JF,Glancy S,Perry E,Nee LD,Hayes PC,Frier BM,Van Look LA,Johnston GI,Reynolds RM,et al. Prevalence of and risk factors for hepatic steatosis and nonalcoholic Fatty liver disease in people with type 2 diabetes: the Edinburgh type 2 diabetes studyDiabetes CareYear: 20113451139114410.2337/dc10-222921478462|
|Thamer C,Machann J,Haap M,Stefan N,Heller E,Schnodt B,Stumvoll M,Claussen C,Fritsche A,Schick F,et al. Intrahepatic lipids are predicted by visceral adipose tissue mass in healthy subjectsDiabetes CareYear: 200427112726272910.2337/diacare.27.11.272615505012|
|Ndumele CE,Nasir K,Conceicao RD,Carvalho JA,Blumenthal RS,Santos RD,Hepatic steatosis, obesity, and the metabolic syndrome are independently and additively associated with increased systemic inflammationArterioscler Thromb Vasc BiolYear: 20113181927193210.1161/ATVBAHA.111.22826221546603|
|Postic C,Girard J,Contribution of de novo fatty acid synthesis to hepatic steatosis and insulin resistance: lessons from genetically engineered miceJ Clin InvestYear: 2008118382983810.1172/JCI3427518317565|
|Fleury C,Neverova M,Collins S,Raimbault S,Champigny O,Levi-Meyrueis C,Bouillaud F,Seldin MF,Surwit RS,Ricquier D,et al. Uncoupling protein-2: a novel gene linked to obesity and hyperinsulinemiaNat GenetYear: 19971532692729054939|
|Thompson MP,Kim D,Links between fatty acids and expression of UCP2 and UCP3 mRNAsFEBS LettYear: 20045681–34915196910|
|Pecqueur C,Bui T,Gelly C,Hauchard J,Barbot C,Bouillaud F,Ricquier D,Miroux B,Thompson CB,Uncoupling protein-2 controls proliferation by promoting fatty acid oxidation and limiting glycolysis-derived pyruvate utilizationFASEB JYear: 200822191817855623|
|Browning JD,Horton JD,Molecular mediators of hepatic steatosis and liver injuryJ Clin InvestYear: 2004114214715215254578|
|Moukdar F,Robidoux J,Lyght O,Pi J,Daniel KW,Collins S,Reduced antioxidant capacity and diet-induced atherosclerosis in uncoupling protein-2-deficient miceJ Lipid ResYear: 2009501597018698091|
|Kohli R,Pan X,Malladi P,Wainwright MS,Whitington PF,Mitochondrial reactive oxygen species signal hepatocyte steatosis by regulating the phosphatidylinositol 3-kinase cell survival pathwayJ Biol ChemYear: 200728229213272133610.1074/jbc.M70175920017540768|
|Vettor R,Fabris R,Serra R,Lombardi AM,Tonello C,Granzotto M,Marzolo MO,Carruba MO,Ricquier D,Federspil G,et al. Changes in FAT/CD36, UCP2, UCP3 and GLUT4 gene expression during lipid infusion in rat skeletal and heart muscleInt J Obes Relat Metab DisordYear: 200226683884712037655|
|Yang QH,Hu SP,Zhang YP,Xie WN,Li N,Ji GY,Qiao NL,Lin XF,Chen TY,Liu HT,Effect of berberine on expressions of uncoupling protein-2 mRNA and protein in hepatic tissue of non-alcoholic fatty liver disease in ratsChin J Integr MedYear: 201117320521110.1007/s11655-011-0668-421359922|
|Chavin KD,Yang S,Lin HZ,Chatham J,Chacko VP,Hoek JB,Walajtys-Rode E,Rashid A,Chen CH,Huang CC,et al. Obesity induces expression of uncoupling protein-2 in hepatocytes and promotes liver ATP depletionJ Biol ChemYear: 199927495692570010.1074/jbc.274.9.569210026188|
|Oh KS,Kim M,Lee J,Kim MJ,Nam YS,Ham JE,Shin SS,Lee CM,Yoon M,Liver PPARalpha and UCP2 are involved in the regulation of obesity and lipid metabolism by swim training in genetically obese db/db miceBiochem Biophys Res CommunYear: 200634531232123910.1016/j.bbrc.2006.04.18216716264|
|Deng S,Yang Y,Han Y,Li X,Wang X,Zhang Z,Wang Y,UCP2 inhibits ROS-mediated apoptosis in A549 under hypoxic conditionsPLoS OneYear: 201271e3071410.1371/journal.pone.003071422292025|
|Diano S,Horvath TL,Mitochondrial uncoupling protein 2 (UCP2) in glucose and lipid metabolismTrends Mol MedYear: 2012181525810.1016/j.molmed.2011.08.00321917523|
|Pecqueur C,Alves-Guerra C,Ricquier D,Bouillaud F,UCP2, a metabolic sensor coupling glucose oxidation to mitochondrial metabolism?IUBMB LifeYear: 200961776276710.1002/iub.18819514063|
|Tordjman K,Standley KN,Bernal-Mizrachi C,Leone TC,Coleman T,Kelly DP,Semenkovich CF,PPARalpha suppresses insulin secretion and induces UCP2 in insulinoma cellsJ Lipid ResYear: 200243693694312032169|
|Ma S,Yang D,Li D,Tang B,Yang Y,Oleic acid induces smooth muscle foam cell formation and enhances atherosclerotic lesion development via CD36Lipids Health DisYear: 2011105310.1186/1476-511X-10-5321486455|
|Ma S,Yang D,Li D,Tang B,Sun M,Yang Y,Cardiac extracellular matrix tenascin-C deposition during fibronectin degradationBiochem Biophys Res CommunYear: 2011409232132710.1016/j.bbrc.2011.05.01321586275|
Previous Document: Dose-dependent effect of antipsychotic drugs on autonomic nervous system activity in schizophrenia.
Next Document: Distribution and preliminary exposure assessment of Bisphenol AF (BPAF) in various environmental mat...