| Inhibition of tumourigenicity of small cell lung cancer cells by suppressing Id3 expression. | |
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MedLine Citation:
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PMID: 20664928 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Id3 is over-expressed in small cell lung cancer (SCLC). To test whether the tumourigenicity of SCLC cells can be inhibited by suppressing Id3 expression, we transfected siRNA into SCLC cell line GLC-19 and established two sublines (G-Id3-1 and G-Id3-7) which expressed only 30% of the level of Id3 measured in control transfectants. Suppression of Id3 expression in both G-Id3-1 and G-Id3-7 cells produced significant reductions in proliferation rates and in numbers of colonies formed in soft agar assay. When G-Id3-1, G-Id3-7 and the control transfectants were inoculated subcutaneously into 3 groups (8 each) of nude mice, respectively, all (100%) inoculated animals produced tumours. Although there was no difference in tumour incidents amongst the 3 groups, significant reductions were observed in both size and weight of tumours produced by either G-Id3-1 or G-Id3-7 cells. While the final average volume of tumours produced in control group was 1012.1+/-394 mm(3), it was significantly reduced (p<0.001, p<0.01) by 2.1- and 2.9-fold to 475.7+/-167 mm(3) and 354.3+/-218 mm(3) in groups inoculated with G-Id3-1 and G-Id3-7 cells, respectively. Similar differences were also observed in average weight of tumours. Upon induction of apoptosis by cytotoxin camptothecin, the percentages of apoptotic cells in G-Id3-1 and G-Id3-7 were, respectively >2.4-fold higher than that in control. The results in this study suggest that highly expressed Id3 in SCLC cells may be an important therapeutic target for tumour suppression. |
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Authors:
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Laleh Kamalian; Shiva S Forootan; Zheng Z Bao; Yu Zhang; John R Gosney; Christopher S Foster; Youqiang Ke |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: International journal of oncology Volume: 37 ISSN: 1791-2423 ISO Abbreviation: Int. J. Oncol. Publication Date: 2010 Sep |
Date Detail:
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Created Date: 2010-07-28 Completed Date: 2010-11-30 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9306042 Medline TA: Int J Oncol Country: Greece |
Other Details:
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Languages: eng Pagination: 595-603 Citation Subset: IM |
Affiliation:
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Molecular Pathology Laboratory, School of Cancer Studies, University of Liverpool, 6th Floor, Duncan Building, Daulby Street, Liverpool, L69 3GA, UK. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Base Sequence Camptothecin / pharmacology Cell Growth Processes / genetics Cell Line, Tumor Gene Expression Regulation, Neoplastic Humans Inhibitor of Differentiation Proteins / antagonists & inhibitors, biosynthesis*, genetics Lung Neoplasms / genetics, metabolism, pathology*, therapy Mice Mice, Nude Molecular Sequence Data Neoplasm Proteins / antagonists & inhibitors, biosynthesis*, genetics RNA, Small Interfering / administration & dosage, genetics Small Cell Lung Carcinoma / genetics, metabolism, pathology*, therapy Transfection |
| Chemical | |
Reg. No./Substance:
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0/Inhibitor of Differentiation Proteins; 0/Neoplasm Proteins; 0/RNA, Small Interfering; 147785-34-0/ID3 protein, human; 7689-03-4/Camptothecin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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