Document Detail


Inhibition of tumourigenicity of small cell lung cancer cells by suppressing Id3 expression.
MedLine Citation:
PMID:  20664928     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Id3 is over-expressed in small cell lung cancer (SCLC). To test whether the tumourigenicity of SCLC cells can be inhibited by suppressing Id3 expression, we transfected siRNA into SCLC cell line GLC-19 and established two sublines (G-Id3-1 and G-Id3-7) which expressed only 30% of the level of Id3 measured in control transfectants. Suppression of Id3 expression in both G-Id3-1 and G-Id3-7 cells produced significant reductions in proliferation rates and in numbers of colonies formed in soft agar assay. When G-Id3-1, G-Id3-7 and the control transfectants were inoculated subcutaneously into 3 groups (8 each) of nude mice, respectively, all (100%) inoculated animals produced tumours. Although there was no difference in tumour incidents amongst the 3 groups, significant reductions were observed in both size and weight of tumours produced by either G-Id3-1 or G-Id3-7 cells. While the final average volume of tumours produced in control group was 1012.1+/-394 mm(3), it was significantly reduced (p<0.001, p<0.01) by 2.1- and 2.9-fold to 475.7+/-167 mm(3) and 354.3+/-218 mm(3) in groups inoculated with G-Id3-1 and G-Id3-7 cells, respectively. Similar differences were also observed in average weight of tumours. Upon induction of apoptosis by cytotoxin camptothecin, the percentages of apoptotic cells in G-Id3-1 and G-Id3-7 were, respectively >2.4-fold higher than that in control. The results in this study suggest that highly expressed Id3 in SCLC cells may be an important therapeutic target for tumour suppression.
Authors:
Laleh Kamalian; Shiva S Forootan; Zheng Z Bao; Yu Zhang; John R Gosney; Christopher S Foster; Youqiang Ke
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  International journal of oncology     Volume:  37     ISSN:  1791-2423     ISO Abbreviation:  Int. J. Oncol.     Publication Date:  2010 Sep 
Date Detail:
Created Date:  2010-07-28     Completed Date:  2010-11-30     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9306042     Medline TA:  Int J Oncol     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  595-603     Citation Subset:  IM    
Affiliation:
Molecular Pathology Laboratory, School of Cancer Studies, University of Liverpool, 6th Floor, Duncan Building, Daulby Street, Liverpool, L69 3GA, UK.
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MeSH Terms
Descriptor/Qualifier:
Animals
Base Sequence
Camptothecin / pharmacology
Cell Growth Processes / genetics
Cell Line, Tumor
Gene Expression Regulation, Neoplastic
Humans
Inhibitor of Differentiation Proteins / antagonists & inhibitors,  biosynthesis*,  genetics
Lung Neoplasms / genetics,  metabolism,  pathology*,  therapy
Mice
Mice, Nude
Molecular Sequence Data
Neoplasm Proteins / antagonists & inhibitors,  biosynthesis*,  genetics
RNA, Small Interfering / administration & dosage,  genetics
Small Cell Lung Carcinoma / genetics,  metabolism,  pathology*,  therapy
Transfection
Chemical
Reg. No./Substance:
0/Inhibitor of Differentiation Proteins; 0/Neoplasm Proteins; 0/RNA, Small Interfering; 147785-34-0/ID3 protein, human; 7689-03-4/Camptothecin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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