| Inhibition of tumorigenicity and enhancement of radiochemosensitivity in head and neck squamous cell cancer-derived ALDH1-positive cells by knockdown of Bmi-1. | |
| | |
MedLine Citation:
|
PMID: 20036608 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
|
Bmi-1, a member of the Polycomb family of transcriptional repressors, is essential for maintaining the self-renewal abilities of adult stem cells. Bmi-1 has been demonstrated to play a role in tumorigenesis in head and neck squamous cell carcinomas (HNSCCs). A recent study has further suggested that ALDH1 may be considered to be a putative marker for HNSCC-derived cancer stem cells. However, the role that Bmi-1 plays in HNSCC-derived ALDH1-positive cells (HNSCC-ALDH1(+)) has yet to be determined. In this study, we demonstrated that HNSCC-ALDH1(+) cells possess tumor initiating properties, are capable of self-renewal, and express higher levels of Bmi-1 as compared to HNSCC-ALDH1(-) cells. To further explore the functional role of Bmi-1 in HNSCC-ALDH1(+) cells, we used a lentiviral vector expressing shRNA to knock down Bmi-1 expression (sh-Bmi-1) in HNSCC-ALDH1(+) cells. Silencing of Bmi-1 significantly enhanced the sensitivity of HNSCC-ALDH1(+) cells to chemoradiation and increased the degree of chemoradiation-mediated apoptosis that occurred. Importantly, knockdown of Bmi-1 increased the effectiveness of radiotherapy and led to the inhibition of tumor growth in nude mice transplanted with HNSCC-ALDH1(+) cells. Kaplan-Meier survival analysis indicated that the mean survival rate of HNSCC-ALDH1(+) tumor-bearing immunocompromised mice treated with radiotherapy was significantly improved by treatment with sh-Bmi-1 as well. In summary, these results suggest that Bmi-1 is a potential target for increasing the sensitivity of HNSCC cancer stem cells to chemoradiotherapy. |
| | |
Authors:
|
Yu-Chih Chen; Charn-Jung Chang; Han-Shui Hsu; Yi-Wei Chen; Lung-Kuo Tai; Ling-Ming Tseng; Guang-Yuh Chiou; Shih-Ching Chang; Shou-Yen Kao; Shih-Hwa Chiou; Wen-Liang Lo |
Related Documents
:
|
16791748 - Evaluation of firefly luciferase bioluminescence mediated photodynamic toxicity in canc... 2671488 - Predominance of squamous cell carcinoma and rarity of adenocarcinoma of maxillary sinus... 12174888 - Increased cyclooxygenase-2 expression in human squamous cell carcinomas of the head and... 7699118 - Keratoacanthoma: a deficient squamous cell carcinoma? study of bcl-2 expression. 19244748 - Protection against hydrogen peroxide-induced cytotoxicity in pc12 cells by guggulsterone. 7626418 - Basic mechanisms of cytodifferentiation and dentinogenesis during dental pulp repair. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-12-29 |
Journal Detail:
|
Title: Oral oncology Volume: 46 ISSN: 1368-8375 ISO Abbreviation: Oral Oncol. Publication Date: 2010 Mar |
Date Detail:
|
Created Date: 2010-02-23 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9709118 Medline TA: Oral Oncol Country: England |
Other Details:
|
Languages: eng Pagination: 158-65 Citation Subset: IM |
Copyright Information:
|
(c) 2009 Elsevier Ltd. All rights reserved. |
Affiliation:
|
Department of Medical Research and Education, Taipei Veterans General Hospital, Taipei 11217, Taiwan. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: A basal-cell-like compartment in head and neck squamous cell carcinomas represents the invasive fron...
Next Document: Head and neck cancer surgery in the elderly--does age influence the postoperative course?