Document Detail


Inhibition of transfected PTEN on human colon cancer.
MedLine Citation:
PMID:  15534929     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
AIM: To study the inhibitory effect of transfected PTEN on LoVo cells. METHODS: Human PTEN cDNA was transferred into LoVo cells via lipofectin and PTEN mRNA levels and its expression were analyzed by Western blot and flow cytometry. Before or after transfection, the effects of 5-Fu on inhibiting cell proliferation and inducing apoptosis were measured by flow cytometry, DNA bands and MTT. RESULTS: PTEN transfection significantly up-regulated PTEN expression in LoVo cells. 5-Fu inhibited cell proliferation and induced apoptosis in transfected LoVo cells. CONCLUSION: Transfected PTEN can remarkably up-regulate PTEN expression in LoVo cells and promote the apoptosis. PTEN transfection is associated with 5-Fu treatment effect and has a cooperatively cytotoxic effect.
Authors:
Shou-Shui Xu; Wen-Lu Shen; Song-Ying Ouyang
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  World journal of gastroenterology : WJG     Volume:  10     ISSN:  1007-9327     ISO Abbreviation:  World J. Gastroenterol.     Publication Date:  2004 Dec 
Date Detail:
Created Date:  2004-11-09     Completed Date:  2005-01-19     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  100883448     Medline TA:  World J Gastroenterol     Country:  China    
Other Details:
Languages:  eng     Pagination:  3670-3     Citation Subset:  IM    
Affiliation:
Second Affiliated Hospital, Shantou University Medical College, Shantou 515031, Guangdong Province, China. smile4612003@yahoo.com.cn
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MeSH Terms
Descriptor/Qualifier:
Antibiotics, Antineoplastic / pharmacology*
Apoptosis / drug effects
Cell Division / drug effects
Colonic Neoplasms*
Fluorouracil / pharmacology*
Gene Expression Regulation, Neoplastic / drug effects*
Humans
PTEN Phosphohydrolase
Phosphoric Monoester Hydrolases / genetics*
Plasmids
RNA, Messenger / metabolism
Transfection
Tumor Cells, Cultured
Tumor Suppressor Proteins / genetics*
Chemical
Reg. No./Substance:
0/Antibiotics, Antineoplastic; 0/RNA, Messenger; 0/Tumor Suppressor Proteins; 51-21-8/Fluorouracil; EC 3.1.3.-/Phosphoric Monoester Hydrolases; EC 3.1.3.48/PTEN protein, human; EC 3.1.3.67/PTEN Phosphohydrolase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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