Document Detail

Inhibition of transfected PTEN on human colon cancer.
MedLine Citation:
PMID:  15534929     Owner:  NLM     Status:  MEDLINE    
AIM: To study the inhibitory effect of transfected PTEN on LoVo cells. METHODS: Human PTEN cDNA was transferred into LoVo cells via lipofectin and PTEN mRNA levels and its expression were analyzed by Western blot and flow cytometry. Before or after transfection, the effects of 5-Fu on inhibiting cell proliferation and inducing apoptosis were measured by flow cytometry, DNA bands and MTT. RESULTS: PTEN transfection significantly up-regulated PTEN expression in LoVo cells. 5-Fu inhibited cell proliferation and induced apoptosis in transfected LoVo cells. CONCLUSION: Transfected PTEN can remarkably up-regulate PTEN expression in LoVo cells and promote the apoptosis. PTEN transfection is associated with 5-Fu treatment effect and has a cooperatively cytotoxic effect.
Shou-Shui Xu; Wen-Lu Shen; Song-Ying Ouyang
Related Documents :
2470149 - Commitment of mouse fibroblasts to adipocyte differentiation by dna transfection.
16776779 - Inactivation of rho gtpases by p190 rhogap reduces human pancreatic cancer cell invasio...
10496649 - Transport and metabolic characterization of caco-2 cells expressing cyp3a4 and cyp3a4 p...
15934299 - Transfection efficiency comparison of oligonucleotide and plasmid to the hl-60 cell lin...
17428869 - Construction and characterization of a full-length infectious simian t-cell lymphotropi...
3459459 - Dna-mediated gene transfer into epidermal cells using electroporation.
8813569 - The effects of cisapride on elemental content in neonatal mouse small intestine in vivo.
16962069 - Angiogenic potential of cd44+ cd90+ multipotent cns stem cells in vitro.
725799 - Effects of addicting and nonaddicting drugs on hela cells.
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  World journal of gastroenterology : WJG     Volume:  10     ISSN:  1007-9327     ISO Abbreviation:  World J. Gastroenterol.     Publication Date:  2004 Dec 
Date Detail:
Created Date:  2004-11-09     Completed Date:  2005-01-19     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  100883448     Medline TA:  World J Gastroenterol     Country:  China    
Other Details:
Languages:  eng     Pagination:  3670-3     Citation Subset:  IM    
Second Affiliated Hospital, Shantou University Medical College, Shantou 515031, Guangdong Province, China.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Antibiotics, Antineoplastic / pharmacology*
Apoptosis / drug effects
Cell Division / drug effects
Colonic Neoplasms*
Fluorouracil / pharmacology*
Gene Expression Regulation, Neoplastic / drug effects*
PTEN Phosphohydrolase
Phosphoric Monoester Hydrolases / genetics*
RNA, Messenger / metabolism
Tumor Cells, Cultured
Tumor Suppressor Proteins / genetics*
Reg. No./Substance:
0/Antibiotics, Antineoplastic; 0/RNA, Messenger; 0/Tumor Suppressor Proteins; 51-21-8/Fluorouracil; EC 3.1.3.-/Phosphoric Monoester Hydrolases; EC protein, human; EC Phosphohydrolase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Intravenous pantoprazole versus ranitidine for prevention of rebleeding after endoscopic hemostasis ...
Next Document:  Serum sIL-2R, TNF-alpha and IFN-gamma in alveolar echinococcosis.