| Inhibition of the synthesis of eicosanoid-like substances in a human oral cancer cell line by interferon-gamma and eicosapentaenoic acid. | |
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MedLine Citation:
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PMID: 9877329 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The objectives were to examine the production of eicosanoids in a Chinese human oral cancer cell line (OEC-M1) and to test the effects of interferon-gamma (IFN-gamma), eicosapentaenoic acid (EPA) and enzyme inhibitors on this biosynthesis. The eicosanoids were identified by reverse phase-high performance liquid chromatography. Two predominant peaks appeared in the chromatograms. One compound (P-1) was identified by ultraviolet absorption at a lambda(max) of 278nm with shoulders at 272 and 284nm. The other compound (P-2) was identified by ultraviolet absorption at a lambda(max) of 284 nm with shoulders at 278 and 290 nm. The production of P- was significantly inhibited by the addition of IFN-gamma (200 and 400 U/ml), and EPA (10 to 40 microM). It was only partially inhibited (p < 0.05) by indomethacin (INDO) (0.5 and 1 microM), nordihydroguaiaretic acid (NDGA) (30 and 60 microM/ml), and eicosa-5,8,11,14-tetraynoic acid (ETYA) (20-60 microM). It was almost completely inhibited by indomethacin (2 and 3 microM), and dexamethasone (0.6 and 6 microM). The production of P-2 was almost completely inhibited by IFN-gamma (200 and 400 U/ml), and partially inhibited (p < 0.05) by EPA (10 and 20 microM), NDGA (30 and 60 microM), ETYA (20 and 40 microM), dexamethasone (0.6 and 6 microM). The production of both peaks was significantly reduced by excluding arachidonic acid (AA), and almost completely inhibited by heating at 100 degrees C for 10 min during incubation. These results demonstrate that two eicosanoid-like compounds are synthesized by the OEC-M cell line and that their production can be modulated by IFN-gamma, EPA, indomethacin, NDGA, ETYA, and dexamethasone. |
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Authors:
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C L Meng; C Y Yang; K L Shen; P Y Wong; H K Lee |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Archives of oral biology Volume: 43 ISSN: 0003-9969 ISO Abbreviation: Arch. Oral Biol. Publication Date: 1998 Dec |
Date Detail:
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Created Date: 1999-03-11 Completed Date: 1999-03-11 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 0116711 Medline TA: Arch Oral Biol Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 979-86 Citation Subset: D; IM |
Affiliation:
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Department of Dentistry, National Defense Medical Center, Taipei, Taiwan, Republic of China. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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5,8,11,14-Eicosatetraynoic Acid
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pharmacology Analysis of Variance Arachidonic Acids / antagonists & inhibitors Carcinoma / metabolism* Chromatography, High Pressure Liquid Cyclooxygenase Inhibitors / pharmacology Dexamethasone / pharmacology Eicosanoids / analysis, antagonists & inhibitors* Eicosapentaenoic Acid / pharmacology* Gingival Neoplasms / metabolism* Glucocorticoids / pharmacology Hot Temperature Humans Indomethacin / pharmacology Interferon-gamma / pharmacology* Nordihydroguaiaretic Acid / pharmacology Spectrophotometry, Ultraviolet Tumor Cells, Cultured |
| Chemical | |
Reg. No./Substance:
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0/Arachidonic Acids; 0/Cyclooxygenase Inhibitors; 0/Eicosanoids; 0/Glucocorticoids; 1191-85-1/5,8,11,14-Eicosatetraynoic Acid; 1553-41-9/Eicosapentaenoic Acid; 50-02-2/Dexamethasone; 500-38-9/Nordihydroguaiaretic Acid; 53-86-1/Indomethacin; 82115-62-6/Interferon-gamma |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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