| Inhibition of sustained hypoxic vasoconstriction by Y-27632 in isolated intrapulmonary arteries and perfused lung of the rat. | |
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MedLine Citation:
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PMID: 10960061 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We have examined the effects of Y-27632, a specific inhibitor of Rho-activated kinases (ROCK I and ROCK II) upon sustained hypoxic pulmonary vasoconstriction (HPV) in both rat isolated small intrapulmonary arteries (IPA) and perfused rat lungs in situ. Y-27632 (100 nM - 3 microM) was found to cause a concentration-dependent inhibition of acute sustained HPV in rat IPA. Application of Y-27632 (10-600 nM) in perfused rat lungs caused no change in basal perfusion pressure, but was found to inhibit HPV in a concentration-dependent manner, resulting in complete ablation of the pressor response to hypoxia at a concentration of 600 nM. Furthermore, addition of Y-27632 at any point during hypoxia caused a reversal of HPV in perfused rat lungs. These results suggest that activation of Rho-associated kinase may be a pivotal step in the generation of sustained HPV. |
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Authors:
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T P Robertson; M Dipp; J P Ward; P I Aaronson; A M Evans |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: British journal of pharmacology Volume: 131 ISSN: 0007-1188 ISO Abbreviation: Br. J. Pharmacol. Publication Date: 2000 Sep |
Date Detail:
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Created Date: 2000-11-03 Completed Date: 2000-11-03 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 7502536 Medline TA: Br J Pharmacol Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 5-9 Citation Subset: IM |
Affiliation:
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Department of Respiratory Medicine and Allergy, GKT School of Medicine, King's College London, Guy's Campus, London SE1 9RT. tom.robertson@kcl.ac.uk |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amides
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pharmacology* Animals Anoxia / physiopathology* Calcium / metabolism Dose-Response Relationship, Drug Enzyme Inhibitors / pharmacology* Intracellular Signaling Peptides and Proteins Lung / drug effects*, physiology Male Perfusion Protein-Serine-Threonine Kinases / antagonists & inhibitors*, physiology Pulmonary Artery / drug effects*, physiology Pyridines / pharmacology* Rats Rats, Wistar Vasoconstriction / drug effects* rho-Associated Kinases |
| Chemical | |
Reg. No./Substance:
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0/Amides; 0/Enzyme Inhibitors; 0/Intracellular Signaling Peptides and Proteins; 0/Pyridines; 138381-45-0/Y 27632; 7440-70-2/Calcium; EC 2.7.11.1/Protein-Serine-Threonine Kinases; EC 2.7.11.1/rho-Associated Kinases |
| Comments/Corrections | |
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