| Inhibition of sulfate transport in Ehrlich ascites tumor cells by 4-acetamido-4'-isothiocyano-stilbene-2,2'-disulfonic acid(SITS). | |
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MedLine Citation:
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PMID: 972169 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The effects of the nonpenetrating amino reactive reagnet 4-acetamido-4'-isothiocyano-stilbene-2-2'-dilsulfonic acid (SITS) on anion transport (sulfate, chloride, and inorganic phosphate) were investigated in Ehrlich ascites tumor cells. Short time exposure to SITS produces a reversible inhibition (92%) of sulfate transport. The kinetics of interaction suggest that reversibly bound SITS competitively inhibits sulfate transport, Ki = 3 X 10(-6)M. Incubation of tumor cells with SITS (1 X 10(-4)M) for longer periods of time results in a time dependent irreversible inhibition of sulfate transport which obeys first order kinetics. The rate coefficient for the inactivation process is 0.040 min-1. The kinetics of irreversible inhibition is best explained by the irreversible binding of SITS to the sulfate transport site, and therefore makes SITS a potentially useful probe for the quantiation of these sites in the tumor cell. The lack of effect of irreversibly bound SITS on either chloride or inorganic phosphate transport points to a specificity in the interaction of SITS with the tumor cell membrane, as well as indicating that an alternate pathway exists for the movement of these anions across the membrane. |
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Authors:
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M L Villereal; C Levinson |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of cellular physiology Volume: 89 ISSN: 0021-9541 ISO Abbreviation: J. Cell. Physiol. Publication Date: 1976 Oct |
Date Detail:
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Created Date: 1976-12-03 Completed Date: 1976-12-03 Revised Date: 2009-10-27 |
Medline Journal Info:
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Nlm Unique ID: 0050222 Medline TA: J Cell Physiol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 303-11 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Biological Transport, Active
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drug effects Cell Line Chlorides / metabolism Depression, Chemical Dose-Response Relationship, Drug Kinetics Phosphates / metabolism Stilbenes / pharmacology* Sulfates / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Chlorides; 0/Phosphates; 0/Stilbenes; 0/Sulfates |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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