| Inhibition studies of soybean (Glycine max) urease with heavy metals, sodium salts of mineral acids, boric acid, and boronic acids. | |
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MedLine Citation:
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PMID: 20014894 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Various inhibitors were tested for their inhibitory effects on soybean urease. The K(i) values for boric acid, 4-bromophenylboronic acid, butylboronic acid, and phenylboronic acid were 0.20 +/- 0.05 mM, 0.22 +/- 0.04 mM, 1.50 +/- 0.10 mM, and 2.00 +/- 0.11 mM, respectively. The inhibition was competitive type with boric acid and boronic acids. Heavy metal ions including Ag(+), Hg(2+), and Cu(2+) showed strong inhibition on soybean urease, with the silver ion being a potent inhibitor (IC(50) = 2.3 x 10(-8) mM). Time-dependent inhibition studies exhibited biphasic kinetics with all heavy metal ions. Furthermore, inhibition studies with sodium salts of mineral acids (NaF, NaCl, NaNO(3), and Na(2)SO(4)) showed that only F(-) inhibited soybean urease significantly (IC(50) = 2.9 mM). Competitive type of inhibition was observed for this anion with a K(i) value of 1.30 mM. |
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Authors:
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Sandeep Kumar; Arvind M Kayastha |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of enzyme inhibition and medicinal chemistry Volume: 25 ISSN: 1475-6374 ISO Abbreviation: J Enzyme Inhib Med Chem Publication Date: 2010 Oct |
Date Detail:
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Created Date: 2010-08-24 Completed Date: 2011-01-04 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101150203 Medline TA: J Enzyme Inhib Med Chem Country: England |
Other Details:
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Languages: eng Pagination: 646-52 Citation Subset: IM |
Affiliation:
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School of Biotechnology, Faculty of science, Banaras Hindu University, Varanasi 221005, India. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Binding, Competitive Boric Acids / chemistry, pharmacology* Boron Compounds / chemistry, pharmacology* Boronic Acids / chemistry, pharmacology Copper / chemistry, pharmacology Enzyme Inhibitors / chemistry, pharmacology* Kinetics Mercury / chemistry, pharmacology Metals, Heavy / chemistry, pharmacology* Seeds / enzymology* Silver / chemistry, pharmacology Sodium Fluoride / chemistry, pharmacology Soybeans / enzymology* Urease / antagonists & inhibitors* |
| Chemical | |
Reg. No./Substance:
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0/Boric Acids; 0/Boron Compounds; 0/Boronic Acids; 0/Enzyme Inhibitors; 0/Metals, Heavy; 11113-50-1/boric acid; 4426-47-5/n-butylboronic acid; 5467-74-3/4-bromophenylboric acid; 7439-97-6/Mercury; 7440-22-4/Silver; 7440-50-8/Copper; 7681-49-4/Sodium Fluoride; 98-80-6/benzeneboronic acid; EC 3.5.1.5/Urease |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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