Document Detail


Inhibition studies of soybean (Glycine max) urease with heavy metals, sodium salts of mineral acids, boric acid, and boronic acids.
MedLine Citation:
PMID:  20014894     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Various inhibitors were tested for their inhibitory effects on soybean urease. The K(i) values for boric acid, 4-bromophenylboronic acid, butylboronic acid, and phenylboronic acid were 0.20 +/- 0.05 mM, 0.22 +/- 0.04 mM, 1.50 +/- 0.10 mM, and 2.00 +/- 0.11 mM, respectively. The inhibition was competitive type with boric acid and boronic acids. Heavy metal ions including Ag(+), Hg(2+), and Cu(2+) showed strong inhibition on soybean urease, with the silver ion being a potent inhibitor (IC(50) = 2.3 x 10(-8) mM). Time-dependent inhibition studies exhibited biphasic kinetics with all heavy metal ions. Furthermore, inhibition studies with sodium salts of mineral acids (NaF, NaCl, NaNO(3), and Na(2)SO(4)) showed that only F(-) inhibited soybean urease significantly (IC(50) = 2.9 mM). Competitive type of inhibition was observed for this anion with a K(i) value of 1.30 mM.
Authors:
Sandeep Kumar; Arvind M Kayastha
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of enzyme inhibition and medicinal chemistry     Volume:  25     ISSN:  1475-6374     ISO Abbreviation:  J Enzyme Inhib Med Chem     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2010-08-24     Completed Date:  2011-01-04     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101150203     Medline TA:  J Enzyme Inhib Med Chem     Country:  England    
Other Details:
Languages:  eng     Pagination:  646-52     Citation Subset:  IM    
Affiliation:
School of Biotechnology, Faculty of science, Banaras Hindu University, Varanasi 221005, India.
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MeSH Terms
Descriptor/Qualifier:
Binding, Competitive
Boric Acids / chemistry,  pharmacology*
Boron Compounds / chemistry,  pharmacology*
Boronic Acids / chemistry,  pharmacology
Copper / chemistry,  pharmacology
Enzyme Inhibitors / chemistry,  pharmacology*
Kinetics
Mercury / chemistry,  pharmacology
Metals, Heavy / chemistry,  pharmacology*
Seeds / enzymology*
Silver / chemistry,  pharmacology
Sodium Fluoride / chemistry,  pharmacology
Soybeans / enzymology*
Urease / antagonists & inhibitors*
Chemical
Reg. No./Substance:
0/Boric Acids; 0/Boron Compounds; 0/Boronic Acids; 0/Enzyme Inhibitors; 0/Metals, Heavy; 11113-50-1/boric acid; 4426-47-5/n-butylboronic acid; 5467-74-3/4-bromophenylboric acid; 7439-97-6/Mercury; 7440-22-4/Silver; 7440-50-8/Copper; 7681-49-4/Sodium Fluoride; 98-80-6/benzeneboronic acid; EC 3.5.1.5/Urease

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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