Document Detail


Inhibition of the streptavidin-biotin interaction by milk.
MedLine Citation:
PMID:  2817396     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Nonfat dried milk is routinely used as a blocking agent and diluent in immunoassays. However, the data presented in this paper demonstrate that milk contains an inhibitor of the biotin-streptavidin interaction. It is recommended that milk be dialyzed or used at lower concentrations when it is employed as a diluent of streptavidin.
Authors:
W L Hoffman; A A Jump
Related Documents :
16899936 - Effects of low-fat milk enriched with phytosterols on plasma cholesterol concentrations...
8298746 - Is prevention of food allergy worthwhile?
9629866 - Chemical and sensory evaluation of vegetable milks from african yam bean sphenostylis s...
10492436 - Comparison of weigh-suckle-weigh and machine milking for measuring ewe milk production.
4029056 - Effects of aerial applications of cypermethrin and demeton-s-methyl on nontarget arthro...
15975676 - Evolution of listeria populations in food samples undergoing enrichment culturing.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Analytical biochemistry     Volume:  181     ISSN:  0003-2697     ISO Abbreviation:  Anal. Biochem.     Publication Date:  1989 Sep 
Date Detail:
Created Date:  1989-11-27     Completed Date:  1989-11-27     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0370535     Medline TA:  Anal Biochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  318-20     Citation Subset:  IM    
Affiliation:
Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas 75235.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Bacterial Proteins / analysis*
Biotin / analysis*
Blotting, Western
Cattle
Chemical Phenomena
Chemistry
Dialysis
Horseradish Peroxidase / antagonists & inhibitors
Indicators and Reagents
Membranes, Artificial
Milk / analysis*
Streptavidin
Grant Support
ID/Acronym/Agency:
AR-36323/AR/NIAMS NIH HHS
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/Indicators and Reagents; 0/Membranes, Artificial; 58-85-5/Biotin; 9013-20-1/Streptavidin; EC 1.11.1.-/Horseradish Peroxidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Reactions of hydroxyamino acids during hydrochloric acid hydrolysis.
Next Document:  Oxazolones as substrates in active site titrations of hydrolases.