Document Detail

Inhibition of the streptavidin-biotin interaction by milk.
MedLine Citation:
PMID:  2817396     Owner:  NLM     Status:  MEDLINE    
Nonfat dried milk is routinely used as a blocking agent and diluent in immunoassays. However, the data presented in this paper demonstrate that milk contains an inhibitor of the biotin-streptavidin interaction. It is recommended that milk be dialyzed or used at lower concentrations when it is employed as a diluent of streptavidin.
W L Hoffman; A A Jump
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Analytical biochemistry     Volume:  181     ISSN:  0003-2697     ISO Abbreviation:  Anal. Biochem.     Publication Date:  1989 Sep 
Date Detail:
Created Date:  1989-11-27     Completed Date:  1989-11-27     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0370535     Medline TA:  Anal Biochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  318-20     Citation Subset:  IM    
Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas 75235.
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MeSH Terms
Bacterial Proteins / analysis*
Biotin / analysis*
Blotting, Western
Chemical Phenomena
Horseradish Peroxidase / antagonists & inhibitors
Indicators and Reagents
Membranes, Artificial
Milk / analysis*
Grant Support
Reg. No./Substance:
0/Bacterial Proteins; 0/Indicators and Reagents; 0/Membranes, Artificial; 58-85-5/Biotin; 9013-20-1/Streptavidin; EC 1.11.1.-/Horseradish Peroxidase

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