Document Detail

Inhibition of single and double-stranded DNA-dependent ATPase of RecA protein by ATP ribose-modified analogs.
MedLine Citation:
PMID:  2956659     Owner:  NLM     Status:  MEDLINE    
The single-stranded (SS) DNA-dependent ATP hydrolysis at pH 7.5 and 6.2 and the double-stranded (DS) DNA-dependent ATP hydrolysis at pH 6.2 by recA protein (no reaction was detectable at pH 7.5) were found to be inhibited competitively by ribose-modified analogs of ATP, 3'-0-anthraniloyl-ATP (Ant-ATP) and 3'-0-(N-methylanthraniloyl)- ATP (Mant-ATP). The Ki values for Ant-ATP and Mant-ATP is SS DNA-dependent hydrolysis were about 8 and 5 microns at pH 7.5 and 12 and 10 microns at pH 6.2. For the DS DNA-dependent hydrolysis, the Ki values for Ant-ATP and Mant-ATP were about 7 and 6 microns. All these Ki values were much smaller than those of ADP which is also a competitive inhibitor for the ATPase activity of the recA protein. Ant-ATP and Mant-ATP caused a reduction in the Hill coefficients for ATP in SS DNA-dependent ATP hydrolysis at pH 7.5 and DS DNA-dependent hydrolysis. These observations showed that the ATP analogs which have a bulky substituent in the ribose moiety of ATP had strong hydrophobic interactions with the ATP binding site on the recA protein and also contributed to the cooperative effect of ATP.
Y Karasaki; H Hirano; K Higashi
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of UOEH     Volume:  9     ISSN:  0387-821X     ISO Abbreviation:  J. UOEH     Publication Date:  1987 Jun 
Date Detail:
Created Date:  1987-09-03     Completed Date:  1987-09-03     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7909645     Medline TA:  J UOEH     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  141-7     Citation Subset:  IM    
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MeSH Terms
Adenosine Triphosphatases / antagonists & inhibitors*
Adenosine Triphosphate / analogs & derivatives*,  pharmacology
Anthranilic Acids / pharmacology*
DNA Helicases*
DNA, Single-Stranded / metabolism*
Hydrogen-Ion Concentration
Rec A Recombinases / metabolism*
Reg. No./Substance:
0/Anthranilic Acids; 0/DNA, Single-Stranded; 56-65-5/Adenosine Triphosphate; 85287-49-6/3'-O-anthraniloyl ATP; 85287-56-5/3'-O-(N-methylanthraniloyl) ATP; EC 2.7.7.-/Rec A Recombinases; EC 3.6.1.-/Adenosine Triphosphatases; EC 3.6.1.-/DNA Helicases

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