Document Detail


Inhibition of sigma-1 receptor reduces N-methyl-D-aspartate induced neuronal injury in methamphetamine-exposed and -naive hippocampi.
MedLine Citation:
PMID:  20600592     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Acute and prolonged methamphetamine (METH) exposure has been reported to moderate the function of N-methyl-d-aspartate type glutamate receptors (NMDAr) in the hippocampus. These effects have been found to be associated with enhanced NMDAr-dependent release of Ca(2+) from IP(3)-sensitive intracellular stores. The present studies were designed to extend these findings and examine the role of the endoplasmic membrane (ER) bound orphan receptor, the sigma-1 receptor, in NMDA-induced neuronal injury and METH withdrawal-potentiated NMDA-induced neuronal injury. Organotypic hippocampal slice cultures were exposed to METH (0 or 100microM) for 6 days and withdrawn for 7 days, then exposed to NMDA (0 or 5microM) for 24h. Additional cultures were also exposed to this regimen and were co-incubated with BD1047 (100microM), a specific inhibitor of ER-bound sigma-1 receptors, for the 24h NMDA exposure. Cytotoxicity was assessed by analysis of propidium iodide uptake. These studies demonstrated that protracted METH exposure and withdrawal significantly potentiated the neuronal injury produced by NMDA exposure. Further, co-exposure to BD1047 with NMDA markedly attenuated neuronal injury in METH-naïve and METH-withdrawn organotypic cultures. As a whole, these data demonstrate that prolonged METH exposure, even at non-toxic concentrations, significantly alters glutamate receptor signaling. Inhibition of sigma-1 receptor-dependent Ca(2+) release from the ER entirely prevented NMDA-induced toxicity in METH-naïve cultures and markedly reduced METH-potentiated toxicity. These findings demonstrate the importance of Ca(2+)-induced intracellular Ca(2+) release in excitotoxic insult and suggest that blockade of glutamatergic overactivity may represent a therapeutic target in the treatment of METH withdrawal.
Authors:
Katherine J Smith; Tracy R Butler; Mark A Prendergast
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2010-06-30
Journal Detail:
Title:  Neuroscience letters     Volume:  481     ISSN:  1872-7972     ISO Abbreviation:  Neurosci. Lett.     Publication Date:  2010 Sep 
Date Detail:
Created Date:  2010-08-09     Completed Date:  2010-11-16     Revised Date:  2013-05-29    
Medline Journal Info:
Nlm Unique ID:  7600130     Medline TA:  Neurosci Lett     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  144-8     Citation Subset:  IM    
Copyright Information:
Published by Elsevier Ireland Ltd.
Affiliation:
Department of Psychology, Spinal Cord and Brain Injury Research Center, University of Kentucky, Lexington, KY 40536-0356, USA. kjsmit@email.unc.edu
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MeSH Terms
Descriptor/Qualifier:
Animals
Calcium / metabolism
Central Nervous System Stimulants / toxicity*
Endoplasmic Reticulum / drug effects,  metabolism
Excitatory Amino Acid Agonists / toxicity
Female
Hippocampus / drug effects,  pathology
Male
Methamphetamine / toxicity*
N-Methylaspartate / toxicity
Neurons / drug effects*,  metabolism,  pathology
Organ Culture Techniques
Rats
Rats, Sprague-Dawley
Receptors, sigma / drug effects,  metabolism*
Substance Withdrawal Syndrome / metabolism*
Grant Support
ID/Acronym/Agency:
T32 DA016176-07/DA/NIDA NIH HHS; T32DA016176/DA/NIDA NIH HHS
Chemical
Reg. No./Substance:
0/Central Nervous System Stimulants; 0/Excitatory Amino Acid Agonists; 0/Receptors, sigma; 0/sigma-1 receptor; 537-46-2/Methamphetamine; 6384-92-5/N-Methylaspartate; 7440-70-2/Calcium
Comments/Corrections

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