Document Detail


Inhibition of Shiga toxin 2 (Stx2) in apple juices and its resistance to pasteurization.
MedLine Citation:
PMID:  20629887     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In the present study, we evaluated Shiga toxin (Stx2) activity in apple juices by measuring a decrease in dehydrogenase activity of Vero cells with the microculture tetrazolium (MTT) assay. Freshly prepared juice from Red Delicious apples and Golden Delicious apples inhibited the biological activity of the bacterial toxin Stx2 produced by E. coli O157:H7 strains. Studies with immunomagnetic beads bearing specific antibodies against the toxin revealed that Stx2 activity was restored when removed from the apple juice. SDS gel electrophoresis revealed no difference (P < 0.05) in the densities or molecular weights between Stx2 in either PBS or apple juices. These results suggest that Stx2 may be reversibly bound to small molecular weight constituents in the juice. The Stx2 toxin was not inactivated on exposure to heat programs (63 degrees C for 30 min, 72 degrees C for 15 s, 89 degrees C for 1 s) commonly used to pasteurize apple juice, but lost all activity when exposed to 100 degrees C for 5 min. The results suggest that pasteurization of apple juice used to inactivate E. coli O157:H7 has no effect on Stx2, and that food-compatible and safe antitoxin compounds can be used to inhibit the biological activity of the Shiga toxin.
Authors:
Reuven Rasooly; Paula M Do; Carol E Levin; Mendel Friedman
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of food science     Volume:  75     ISSN:  1750-3841     ISO Abbreviation:  J. Food Sci.     Publication Date:  2010 Jun 
Date Detail:
Created Date:  2010-07-15     Completed Date:  2010-10-20     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0014052     Medline TA:  J Food Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  M296-301     Citation Subset:  IM    
Affiliation:
Foodborne Contaminants Research Units, Western Regional Research Center, Agricultural Research Service, U.S. Dept. of Agriculture, Albany, CA, USA.
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MeSH Terms
Descriptor/Qualifier:
Beverages / microbiology*
Colony Count, Microbial
Escherichia coli / immunology,  metabolism
Food Microbiology*
Food Preservation / methods*
Hot Temperature*
Malus / microbiology*
Molecular Weight
Oxidoreductases / metabolism
Shiga Toxin 2 / analysis*,  antagonists & inhibitors*,  chemistry,  immunology
Chemical
Reg. No./Substance:
0/Shiga Toxin 2; EC 1.-/Oxidoreductases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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