| Inhibition of restriction endonuclease cleavage site via triple helix formation by homopyrimidine phosphorothioate oligonucleotides. | |
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MedLine Citation:
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PMID: 8240378 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The ability of pyrimidine rich oligonucleotide phosphorothioate to form stable triple helical structures with the sequence containing the recognition site for the class II-S restriction enzyme Ksp 632-I was examined. First, we synthesized double strand oligonucleotides corresponding to the SV40 sites and studied their interaction with homopyrimidine oligodeoxyribonucleotides including replacement of the other chain either PS group (SO-ODNs) in second nucleotide position (from 5'-terminus) and end capped with the PS group at both 3'- and 5'-ends (S2O-ODNs). The resulting perfect DNA triplexes were detected by gel-mobility shift. The phosphorothioate oligonucleotide analogues (SO-ODNs) and (S2O-ODNs) were shown to inhibit enzymatic cleavage under conditions that allow for triple helix formation. Inhibition is sequence-specific and occurs in the micromolar concentration range. Of particular interest is the Sp-phosphorothioate analogue (Sp-SO-ODNs) which inhibited endonuclease more than the other phosphorothioate oligonucleotide analogues (Rp-SO-ODNs or S2O-ODNs). |
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Authors:
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S Tsukahara; S G Kim; H Takaku |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biochemical and biophysical research communications Volume: 196 ISSN: 0006-291X ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 1993 Oct |
Date Detail:
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Created Date: 1993-12-09 Completed Date: 1993-12-09 Revised Date: 2008-08-26 |
Medline Journal Info:
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Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 990-6 Citation Subset: IM |
Affiliation:
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Department of Industrial Chemistry, Chiba Institute of Technology, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Base Sequence Deoxyribonucleases, Type II Site-Specific / antagonists & inhibitors* Molecular Sequence Data Oligodeoxyribonucleotides / pharmacology* Pyrimidines Structure-Activity Relationship Substrate Specificity Thionucleotides / pharmacology* |
| Chemical | |
Reg. No./Substance:
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0/Oligodeoxyribonucleotides; 0/Pyrimidines; 0/Thionucleotides; EC 3.1.21.4/CCGCGG-specific type II deoxyribonucleases; EC 3.1.21.4/Deoxyribonucleases, Type II Site-Specific |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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