| Inhibition of receptor-mediated apoptosis upon Bcl-2 overexpression is not associated with increased antioxidant status. | |
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MedLine Citation:
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PMID: 18687309 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Bcl-2 is reported to augment the antioxidant capacity of cells and this is hypothesized to contribute to the anti-apoptotic activity of this oncoprotein. We generated a number of stable Jurkat cell lines expressing varying levels of Bcl-2, and showed a strong correlation between Bcl-2 levels and resistance to Fas-mediated apoptosis. While individual differences could be detected, there was no overall correlation between Bcl-2 and the expression and activity of superoxide dismutases, catalase, glutathione peroxidases, thioredoxin, thioredoxin reductases, and peroxiredoxins. Cells transfected with Bcl-2 averaged 70% more glutathione than parental cells, but there was no correlation between glutathione and resistance to apoptosis. This challenges the hypothesis that the anti-apoptotic properties of Bcl-2 are linked to a global increase in antioxidant status. |
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Authors:
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Susan J Thomson; Andrew G Cox; Sarah L Cuddihy; Juliet M Pullar; Mark B Hampton |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-08-05 |
Journal Detail:
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Title: Biochemical and biophysical research communications Volume: 375 ISSN: 1090-2104 ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 2008 Oct |
Date Detail:
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Created Date: 2008-09-01 Completed Date: 2008-09-09 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: United States |
Other Details:
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Languages: eng Pagination: 145-50 Citation Subset: IM |
Affiliation:
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Free Radical Research Group, Department of Pathology, University of Otago, P.O. Box 4345, Christchurch, New Zealand. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Antigens, CD95
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metabolism Antioxidants / metabolism* Apoptosis* Glutathione / metabolism Humans Hydrogen Peroxide / toxicity Jurkat Cells Oxidoreductases / metabolism* Proto-Oncogene Proteins c-bcl-2 / biosynthesis*, genetics Transfection |
| Chemical | |
Reg. No./Substance:
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0/Antigens, CD95; 0/Antioxidants; 0/Proto-Oncogene Proteins c-bcl-2; 70-18-8/Glutathione; 7722-84-1/Hydrogen Peroxide; EC 1.-/Oxidoreductases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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