Document Detail


Inhibition of purine phosphoribosyltransferases from Ehrlich ascites-tumour cells by purine nucleotides.
MedLine Citation:
PMID:  5969281     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
1. The purine bases adenine, hypoxanthine and guanine were rapidly incorporated into the nucleotide fraction of Ehrlich ascites-tumour cells in vivo. 2. The reaction of 5'-phosphoribosyl pyrophosphate with adenine phosphoribosyltransferase from ascites-tumour cells (K(m) 6.5-11.9mum) was competitively inhibited by AMP, ADP, ATP and GMP (K(i) 7.5, 21.9, 395 and 118mum respectively). Similarly the reactions of 5'-phosphoribosyl pyrophosphate with both hypoxanthine phosphoribosyltransferase and guanine phosphoribosyltransferase (K(m) 18.4-31 and 37.6-44.2mum respectively) were competitively inhibited by IMP (K(i) 52 and 63.5mum) and by GMP (K(i) 36.5 and 5.9mum). 3. The nucleotides tested as inhibitors did not appreciably compete with the purine bases in the phosphoribosyltransferase reactions. 4. It was postulated that the purine phosphoribosyltransferases of Ehrlich ascites-tumour cells may be effectively separated from the adenine nucleotide pool of these cells.
Authors:
A W Murray
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Biochemical journal     Volume:  100     ISSN:  0264-6021     ISO Abbreviation:  Biochem. J.     Publication Date:  1966 Sep 
Date Detail:
Created Date:  1967-08-26     Completed Date:  1967-08-26     Revised Date:  2010-09-13    
Medline Journal Info:
Nlm Unique ID:  2984726R     Medline TA:  Biochem J     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  671-4     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adenine Nucleotides / pharmacology*
Animals
Carbon Isotopes
Carcinoma, Ehrlich Tumor / metabolism*
Female
Glucosyltransferases / metabolism*
Guanine Nucleotides / pharmacology*
Hypoxanthines / pharmacology*
Kinetics
Mice
Nucleotides / pharmacology*
Chemical
Reg. No./Substance:
0/Adenine Nucleotides; 0/Carbon Isotopes; 0/Guanine Nucleotides; 0/Hypoxanthines; 0/Nucleotides; EC 2.4.1.-/Glucosyltransferases
Comments/Corrections

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