Document Detail

Inhibition of purine phosphoribosyltransferases from Ehrlich ascites-tumour cells by purine nucleotides.
MedLine Citation:
PMID:  5969281     Owner:  NLM     Status:  MEDLINE    
1. The purine bases adenine, hypoxanthine and guanine were rapidly incorporated into the nucleotide fraction of Ehrlich ascites-tumour cells in vivo. 2. The reaction of 5'-phosphoribosyl pyrophosphate with adenine phosphoribosyltransferase from ascites-tumour cells (K(m) 6.5-11.9mum) was competitively inhibited by AMP, ADP, ATP and GMP (K(i) 7.5, 21.9, 395 and 118mum respectively). Similarly the reactions of 5'-phosphoribosyl pyrophosphate with both hypoxanthine phosphoribosyltransferase and guanine phosphoribosyltransferase (K(m) 18.4-31 and 37.6-44.2mum respectively) were competitively inhibited by IMP (K(i) 52 and 63.5mum) and by GMP (K(i) 36.5 and 5.9mum). 3. The nucleotides tested as inhibitors did not appreciably compete with the purine bases in the phosphoribosyltransferase reactions. 4. It was postulated that the purine phosphoribosyltransferases of Ehrlich ascites-tumour cells may be effectively separated from the adenine nucleotide pool of these cells.
A W Murray
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Biochemical journal     Volume:  100     ISSN:  0264-6021     ISO Abbreviation:  Biochem. J.     Publication Date:  1966 Sep 
Date Detail:
Created Date:  1967-08-26     Completed Date:  1967-08-26     Revised Date:  2010-09-13    
Medline Journal Info:
Nlm Unique ID:  2984726R     Medline TA:  Biochem J     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  671-4     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Adenine Nucleotides / pharmacology*
Carbon Isotopes
Carcinoma, Ehrlich Tumor / metabolism*
Glucosyltransferases / metabolism*
Guanine Nucleotides / pharmacology*
Hypoxanthines / pharmacology*
Nucleotides / pharmacology*
Reg. No./Substance:
0/Adenine Nucleotides; 0/Carbon Isotopes; 0/Guanine Nucleotides; 0/Hypoxanthines; 0/Nucleotides; EC 2.4.1.-/Glucosyltransferases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  The effect of carbon tetrachloride on the lipoproteins of rat-liver cell sap and serum.
Next Document:  Studies on the biochemistry of Penicillium charlesii. Influence of various dicarboxylic acids on gal...