Document Detail

Inhibition of protein synthesis and cell proliferation in cultured human breast cancer cells treated with mitoxantrone.
MedLine Citation:
PMID:  6498809     Owner:  NLM     Status:  MEDLINE    
Mitoxantrone suppresses cell proliferation, inhibits protein synthesis and induces ultrastructural alterations in the T-47D and MDA-MB-231 breast cancer cell lines. After 24 h treatment with 10(-9), 10(-7) and 10(-5) M drug and 8 h incubation with [35S]methionine, protein synthesis declined rapidly. While a 10-15% decrease in protein synthesis at 10(-9) M was observed, more than 95% inhibition of protein synthesis occurred at 10(-5) M mitoxantrone in both cell lines. Sodium dodecylsulfate (SDS) gel electrophoresis of labeled proteins revealed no qualitative changes in either cell line. However, only trace amounts of several proteins were present in T-47D cells treated with 10(-5) M drug. At 10(-9) M mitoxantrone had little effect on cell proliferation. At 10(-7) M, 25% and 35% growth inhibition in T-47D and MDA-MB-231 cells was observed, respectively. Cell growth at 10(-5) M was abolished. Cytotoxicity was evident at drug concentrations above 10(-5) M. Ultrastructural alterations in the nucleoli of both cell lines included disintegration and segregation of granular and fibrillar components and the disappearance of nucleolar organizers at 10(-7)-10(-5) M mitoxantrone.
A R Safa; M T Tseng
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cancer letters     Volume:  24     ISSN:  0304-3835     ISO Abbreviation:  Cancer Lett.     Publication Date:  1984 Oct 
Date Detail:
Created Date:  1984-12-28     Completed Date:  1984-12-28     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7600053     Medline TA:  Cancer Lett     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  317-26     Citation Subset:  IM    
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MeSH Terms
Anthraquinones / pharmacology*
Antineoplastic Agents / pharmacology*
Breast Neoplasms / metabolism*,  pathology,  ultrastructure
Cell Division / drug effects
Cell Nucleolus / drug effects
Cells, Cultured
Dose-Response Relationship, Drug
Electrophoresis, Polyacrylamide Gel
Neoplasm Proteins / biosynthesis*
Reg. No./Substance:
0/Anthraquinones; 0/Antineoplastic Agents; 0/Neoplasm Proteins; 65271-80-9/Mitoxantrone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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