| Inhibition of poly(adp-ribose) polymerase reduces cardiomyocytic apoptosis after global cardiac arrest under cardiopulmonary bypass. | |
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MedLine Citation:
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PMID: 16525356 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Cardiomyocytic apoptosis occurs after cardiopulmonary bypass (CPB) despite the use of perfusion techniques and cardioplegic solutions. Reactive oxygen species (ROS) cause single-strand DNA breaks and activate nuclear poly(ADP-ribose) polymerase (PARP), which leads to cellular damage. Therefore, the inhibition of PARP might protect cardiomyocytes from oxidative injuries. In this study, experiments were designed to determine whether a PARP inhibitor could decrease the myocardial ischemia/reperfusion injury after cardioplegia-induced global cardiac arrest under CPB, attenuate the appearance of cardiomyocytic apoptosis, and decrease damage from ROS. New Zealand white rabbits (10 in each group) were subjected to total CPB. Rabbits were weaned from CPB and reperfused for 4 h before the hearts were harvested. 3-Aminobenzamide and/or 3-aminobenzoic acid was added to the cardioplegic solution. The ascending aorta was cross-clamped for 60 min while intermittent cold crystalloid cardioplegic solution was infused into the aortic root every 20 min. The reperfused hearts were harvested and studied for evidence of apoptosis using the TUNEL method and Western blot analyses. The oxidative insults were checked using ELISA to detect plasma isoprostane and cytokines levels. The occurrence of cardiomyocytic apoptosis was significantly less in PARP inhibitor recipients than in PARP-inhibitor-naive controls. Plasma isoprostane and various cytokines were significantly elevated in PARP-inhibitor-naive controls but significantly reduced in PARP inhibitor recipients. Western blot analysis revealed similar patterns. PARP inhibitor-supplemented crystalloid cardioplegic solution diminished postischemic cardiomyocytic apoptosis and ROS-mediated injuries after global cardiac arrest under CPB, possibly via inhibiting both caspase-dependent and -independent apoptotic pathways, which also preserved postischemic myocardial contractility. |
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Authors:
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Chi-Hsiao Yeh; Tzu-Ping Chen; Chieh-Hung Lee; Yi-Chen Wu; Yu-Min Lin; Pyng Jing Lin |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Shock (Augusta, Ga.) Volume: 25 ISSN: 1073-2322 ISO Abbreviation: Shock Publication Date: 2006 Feb |
Date Detail:
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Created Date: 2006-03-09 Completed Date: 2006-07-18 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9421564 Medline TA: Shock Country: United States |
Other Details:
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Languages: eng Pagination: 168-75 Citation Subset: IM |
Affiliation:
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Division of Thoracic & Cardiovascular Surgery, Chang Gung Memorial Hospital, 222 Mai-Chin Road, Keelung, Taiwan 204. yehccl@cgmh.org.tw |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Aminobenzoic Acids
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administration & dosage* Animals Apoptosis / drug effects* Benzamides / administration & dosage* Cardioplegic Solutions / administration & dosage Cardiopulmonary Bypass Enzyme Inhibitors / administration & dosage* Heart Arrest / drug therapy, enzymology*, pathology Male Myocytes, Cardiac / enzymology*, pathology Poly(ADP-ribose) Polymerases / antagonists & inhibitors*, metabolism Rabbits Reperfusion Injury / drug therapy, enzymology, pathology |
| Chemical | |
Reg. No./Substance:
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0/Aminobenzoic Acids; 0/Benzamides; 0/Cardioplegic Solutions; 0/Enzyme Inhibitors; 3544-24-9/3-aminobenzamide; 99-05-8/3-aminobenzoic acid; EC 2.4.2.30/Poly(ADP-ribose) Polymerases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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