| Inhibition of poly(ADP-ribose) polymerase prevents allergen-induced asthma-like reaction in sensitized Guinea pigs. | |
| | |
MedLine Citation:
|
PMID: 15254147 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Poly(ADP-ribose) polymerase (PARP) plays an important role in tissue injury in conditions associated with oxidative stress and inflammation. Because asthma is a chronic inflammatory disorder of the airways, we designed the present experimental study to evaluate the effects of PARP inhibition on allergen-induced asthma-like reaction in ovalbumin-sensitized guinea pigs. Cough and dyspnea in response to ovalbumin aerosol were absent in naive guinea pigs, whereas they became severe in the sensitized animals. In the latter ones, ovalbumin aerosol also induced a rapid increase in PARP activity, bronchiolar constriction, pulmonary air space inflation, mast cell degranulation, poly(ADP-ribose) and nitrotyrosine immunostaining, myeloperoxidase activity, and malondialdehyde in lung tissue, as well as a rise in the amounts of nitrites and tumor necrosis factor-alpha in bronchoalveolar lavage fluid. Pretreatment with the PARP inhibitors 3-aminobenzamide (10 mg/kg b.wt.) or 5-aminoisoquinolinone (0.5 mg/kg b.wt.) given i.p. 3 h before ovalbumin challenge significantly reduced the severity of cough and the occurrence of dyspnea and delayed the onset of respiratory abnormalities. Both PARP inhibitors were also able to prevent the above morphological and biochemical changes of lung tissue or bronchoalveolar lavage fluid induced by ovalbumin challenge. Conversely, p-aminobenzoic acid, the inactive analog of 3-aminobenzamide, had no effects. |
| | |
Authors:
|
Ylenia Suzuki; Emanuela Masini; Cosimo Mazzocca; Salvatore Cuzzocrea; Anna Ciampa; Hisanori Suzuki; Daniele Bani |
Related Documents
:
|
17615687 - Nanoparticle realgar powders induce apoptosis in u937 cells through caspase mapk and mi... 167107 - Are cytotoxicity and interferon inducing activity of poly(i).poly(c) invariably linked ... 17714487 - Caspase-dependent apoptosis of the hct-8 epithelial cell line induced by the parasite g... 12175527 - Se-methylselenocysteine induces apoptosis through caspase activation and bax cleavage m... 10637267 - A single amino acid determines the immunostimulatory activity of interleukin 10. 10190187 - Effects of shiitake (lentinus edodes) extract on human neutrophils and the u937 monocyt... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2004-07-13 |
Journal Detail:
|
Title: The Journal of pharmacology and experimental therapeutics Volume: 311 ISSN: 0022-3565 ISO Abbreviation: J. Pharmacol. Exp. Ther. Publication Date: 2004 Dec |
Date Detail:
|
Created Date: 2004-11-23 Completed Date: 2005-01-13 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 0376362 Medline TA: J Pharmacol Exp Ther Country: United States |
Other Details:
|
Languages: eng Pagination: 1241-8 Citation Subset: IM |
Affiliation:
|
Department of Anatomy, Histology, and Forensic Medicine, Section of Histology, University of Florence, Viale G. Pieraccini, 6, I-50139 Florence, Italy. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Allergens* Animals Anti-Asthmatic Agents* Asthma / physiopathology, prevention & control* Benzamides / pharmacology Bronchoalveolar Lavage Fluid / chemistry Enzyme Inhibitors / pharmacology* Guinea Pigs Immunohistochemistry Isoquinolines / pharmacology Lung / pathology Male Malondialdehyde / metabolism Mast Cells / drug effects, metabolism Nitric Oxide / metabolism Ovalbumin / immunology Peroxidase / metabolism Poly(ADP-ribose) Polymerases / antagonists & inhibitors* Respiratory Mechanics / drug effects, physiology Tumor Necrosis Factor-alpha / metabolism Tyrosine / analogs & derivatives*, pharmacology |
| Chemical | |
Reg. No./Substance:
|
0/5-aminoisoquinolinone; 0/Allergens; 0/Anti-Asthmatic Agents; 0/Benzamides; 0/Enzyme Inhibitors; 0/Isoquinolines; 0/Tumor Necrosis Factor-alpha; 10102-43-9/Nitric Oxide; 3544-24-9/3-aminobenzamide; 3604-79-3/3-nitrotyrosine; 542-78-9/Malondialdehyde; 55520-40-6/Tyrosine; 9006-59-1/Ovalbumin; EC 1.11.1.7/Peroxidase; EC 2.4.2.30/Poly(ADP-ribose) Polymerases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Interaction between dinucleotide and nicotinic receptors in individual cholinergic terminals.
Next Document: Hyperpolarized shifts in the voltage dependence of fast inactivation of Nav1.4 and Nav1.5 in a rat m...