| Inhibition of poly(ADP-ribose) polymerase as a possible reason for activation of Ca2+/Mg2+-dependent endonuclease in thymocytes of irradiated rats. | |
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MedLine Citation:
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PMID: 3129376 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The molecular mechanism of activation of Ca2+/Mg2+-dependent endonuclease in thymocytes of irradiated rats was studied. Thymocyte nuclei of control and irradiated rats were pre-incubated with NAD under conditions favourable for poly ADP-ribosylation. Pre-incubation results in a decrease in the rate of autolytic DNA digestion by Ca2+/Mg2+-dependent endonuclease of 6-7- and 2-3-fold for control and irradiated animals, respectively. The activity of Ca2+/Mg2+-nuclease extracted from the nuclei pre-incubated with NAD is also considerably decreased. The presence of nicotinamide and thymidine in the preincubation medium prevents the suppression of Ca2+/Mg2+-nuclease activity. In the experiments performed with isolated nuclei and permeabilized thymocytes the synthesis of poly(ADP-ribose) does not significantly change within 1 h after irradiation at a dose of 10 Gy, whereas 2 and 3 h after the exposure it decreases by 35-40 and 45-55 per cent, respectively. The activity of poly(ADP-ribose) glycohydrolase in this period is similar to that in the controls. The average size of the de novo synthesized chains of poly(ADP-ribose) increases from 11 to 17 ADP-ribose units by the second hour after irradiation. Inhibition of poly(ADP-ribose) polymerase in the postirradiation period preceded the internucleosomal fragmentation of chromatin. The results suggest that activation of Ca2+/Mg2+-nuclease in irradiated thymocytes is accounted for by the disturbance of its poly ADP-ribosylation. |
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Authors:
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P A Nelipovich; L V Nikonova; S R Umansky |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: International journal of radiation biology and related studies in physics, chemistry, and medicine Volume: 53 ISSN: 0020-7616 ISO Abbreviation: Int. J. Radiat. Biol. Relat. Stud. Phys. Chem. Med. Publication Date: 1988 May |
Date Detail:
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Created Date: 1988-06-09 Completed Date: 1988-06-09 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0374725 Medline TA: Int J Radiat Biol Relat Stud Phys Chem Med Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 749-65 Citation Subset: IM |
Affiliation:
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Institute of Biological Physics, USSR Academy of Sciences, Moscow Region. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Nucleus / enzymology DNA Damage Endodeoxyribonucleases / metabolism* Enzyme Activation Male NAD / metabolism Poly(ADP-ribose) Polymerases / antagonists & inhibitors* Rats Rats, Inbred Strains Thymus Gland / enzymology, radiation effects* |
| Chemical | |
Reg. No./Substance:
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53-84-9/NAD; EC 2.4.2.30/Poly(ADP-ribose) Polymerases; EC 3.1.-/Endodeoxyribonucleases; EC 3.1.21.-/calcium magnesium dependent endodeoxyribonuclease |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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