| Inhibition of platelet aggregation and thromboxane production by low concentrations of aspirin in vitro. | |
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MedLine Citation:
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PMID: 3370916 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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1. The aspirin concentrations previously reported to inhibit platelet aggregation in vitro (40-500 mumol/l) are much greater than those required in vivo in man (5 mumol/l). 2. Human platelet-rich plasma was incubated with buffer or various aspirin concentrations at 37 degrees C for up to 4.5 h. Platelet aggregation and thromboxane generation were measured in response to collagen (0.4-6.3 micrograms/ml) and adenosine 5'-pyrophosphate (0.5-4 mumol/l). 3. The concentration of aspirin needed to inhibit platelet aggregation in response to a critical concentration of aggregating agent (lowest concentration to cause greater than 50% aggregation) was lower than that required for higher concentrations of aggregating agent. 4. With more prolonged incubation times with aspirin, lower concentrations of aspirin inhibited platelet aggregation. 5. Inhibition of platelet aggregation and thromboxane formation by 10 mumol/l aspirin was maximal by 90 min. There was progressive inhibition by 3 mumol/l aspirin during incubation for 270 min. By the end of this time there was also significant inhibition by 1 mumol/l aspirin. 6. The apparent discrepancy between inhibitory aspirin concentrations in vivo and those observed in vitro in previous studies appears to have been resolved by extending the incubation time of platelets with low aspirin concentrations, thus mimicking the conditions in vivo. |
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Authors:
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D Sils; S E Rodgers; J V Lloyd; K M Wilson; D M Siebert; F Bochner |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Clinical science (London, England : 1979) Volume: 74 ISSN: 0143-5221 ISO Abbreviation: Clin. Sci. Publication Date: 1988 May |
Date Detail:
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Created Date: 1988-07-08 Completed Date: 1988-07-08 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7905731 Medline TA: Clin Sci (Lond) Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 491-7 Citation Subset: IM |
Affiliation:
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Department of Clinical and Experimental Pharmacology, University of Adelaide, Australia. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Aspirin
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administration & dosage,
pharmacology* Blood Platelets / drug effects*, metabolism Collagen / antagonists & inhibitors Dose-Response Relationship, Drug Humans Platelet Aggregation / drug effects* Thromboxanes / biosynthesis* |
| Chemical | |
Reg. No./Substance:
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0/Thromboxanes; 50-78-2/Aspirin; 9007-34-5/Collagen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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