| Inhibition of pathogen-induced apoptosis by a Coxiella burnetii type IV effector protein. | |
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MedLine Citation:
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PMID: 20944063 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Coxiella burnetii and Legionella pneumophila are evolutionarily related pathogens with different intracellular infection strategies. C. burnetii persists within and is transmitted by mammalian hosts, whereas, L. pneumophila is found primarily in the environment associated with protozoan hosts. Although a type IV secretion system encoded by the defect in organelle trafficking (dot) and intracellular multiplication (icm) genes is a virulence determinant that remains highly conserved in both bacteria, the two pathogens encode a different array of effector proteins that are delivered into host cells by the Dot/Icm machinery. This difference suggests that adaptations to evolutionarily distinct hosts may be reflected in the effector protein repertoires displayed by these two pathogens. Here we provide evidence in support of this hypothesis. We show that a unique C. burnetii effector from the ankyrin repeat (Ank) family called AnkG interferes with the mammalian apoptosis pathway. AnkG was found to interact with the host protein gC1qR (p32). Either the addition of AnkG to the repertoire of L. pneumophila effector proteins or the silencing of p32 in mouse dendritic cells resulted in a gain of function that allowed intracellular replication of L. pneumophila in these normally restrictive mammalian host cells by preventing rapid pathogen-induced apoptosis. These data indicate that p32 regulates pathogen-induced apoptosis and that AnkG functions to block this pathway. Thus, emergence of an effector protein that interferes with a proapoptotic signaling pathway directed against intracellular bacteria correlates with adaptation of a pathogen to mammalian hosts. |
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Authors:
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Anja Lührmann; Catarina V Nogueira; Kimberly L Carey; Craig R Roy |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2010-10-13 |
Journal Detail:
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Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 107 ISSN: 1091-6490 ISO Abbreviation: Proc. Natl. Acad. Sci. U.S.A. Publication Date: 2010 Nov |
Date Detail:
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Created Date: 2010-11-03 Completed Date: 2010-11-30 Revised Date: 2011-11-03 |
Medline Journal Info:
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Nlm Unique ID: 7505876 Medline TA: Proc Natl Acad Sci U S A Country: United States |
Other Details:
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Languages: eng Pagination: 18997-9001 Citation Subset: IM |
Affiliation:
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Section of Microbial Pathogenesis, Yale University School of Medicine, New Haven, CT 06536, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Motifs Animals Antigens, CD44 / genetics, immunology*, metabolism Apoptosis / genetics, immunology* Bacterial Proteins / genetics, immunology*, metabolism Coxiella burnetii / pathogenicity, physiology* Dendritic Cells / immunology, microbiology HEK293 Cells Host-Pathogen Interactions / immunology* Humans Legionella pneumophila / pathogenicity, physiology* Mice Q Fever / genetics, immunology*, metabolism |
| Grant Support | |
ID/Acronym/Agency:
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AI066547/AI/NIAID NIH HHS; R01 AI048770-12/AI/NIAID NIH HHS; R01 AI064559-05/AI/NIAID NIH HHS; R01-AI048770/AI/NIAID NIH HHS; R01-AI064559/AI/NIAID NIH HHS; U54-AI057158-LIPKIN/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Antigens, CD44; 0/Bacterial Proteins; 0/C1qbp protein, mouse |
| Comments/Corrections | |
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