Document Detail

Inhibition of osteopontin expression and function in oral cancer cell lines by antisense oligonucleotides.
MedLine Citation:
PMID:  15596299     Owner:  NLM     Status:  MEDLINE    
We examined expression and function of osteopontin (OPN) in oral cancer cell lines using antisense oligonucleotide (AS). Quantitative real-time RT-PCR showed that expression in BSC-OF cells was significantly higher (10-fold) than that in KB cell. AS-study showed that foci of AS-treated BSC-OF cells possessed thin processes and radiated morphologically, although BSC-OF cells showed round foci. Cell growth in AS-group was lower (<80%) than the control. Invasion ability in AS-group became significantly lower (P<0.01). These results suggest that BSC-OF cell is useful for over-expression of OPN, and that OPN contributes to morphology, growth and invasion.
Takashi Muramatsu; Kaori Shima; Kazumasa Ohta; Harutoshi Kizaki; Yasufumi Ro; Yohko Kohno; Yoshihiro Abiko; Masaki Shimono
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cancer letters     Volume:  217     ISSN:  0304-3835     ISO Abbreviation:  Cancer Lett.     Publication Date:  2005 Jan 
Date Detail:
Created Date:  2004-12-14     Completed Date:  2005-03-03     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7600053     Medline TA:  Cancer Lett     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  87-95     Citation Subset:  IM    
Department of Pathology, Tokyo Dental College, 1-2-2, Masago, Mihama-ku, Chiba 261-8502, Japan.
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MeSH Terms
Blotting, Western
Cell Line, Tumor
Cell Proliferation / drug effects
Mouth Neoplasms / metabolism*
Neoplasm Invasiveness*
Oligonucleotides, Antisense*
Reverse Transcriptase Polymerase Chain Reaction
Sialoglycoproteins / biosynthesis*,  immunology
Reg. No./Substance:
0/Oligonucleotides, Antisense; 0/SPP1 protein, human; 0/Sialoglycoproteins; 106441-73-0/Osteopontin

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