| Inhibition of ocular angiogenesis by diced small interfering RNAs (siRNAs) specific to vascular endothelial growth factor (VEGF). | |
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MedLine Citation:
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PMID: 16500768 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The effects of diced small interfering RNAs (siRNAs) designed for vascular endothelial growth factor (VEGF) on the expression of VEGF in human retinal pigment epithelial cell line ARPE-19 cells in vitro and on corneal angiogenesis in vivo were examined. The exposure to diced siRNAs significantly reduced the VEGF mRNA expression in ARPE-19 cells with minimal toxicity. In suture-induced corneal angiogenesis models, diced siRNAs minimized the severity of angiogenesis. Histological analysis displayed no particular tissue damage in the conjunctiva where siRNA was injected. The approach using diced siRNAs can be a new tool for various neovascular ocular diseases. |
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Authors:
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Masatoshi Murata; Takako Takanami; Shinji Shimizu; Yosiko Kubota; Saburo Horiuchi; Wataru Habano; Jian-xing Ma; Sanai Sato |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Current eye research Volume: 31 ISSN: 0271-3683 ISO Abbreviation: Curr. Eye Res. Publication Date: 2006 Feb |
Date Detail:
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Created Date: 2006-02-27 Completed Date: 2006-04-18 Revised Date: 2007-05-02 |
Medline Journal Info:
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Nlm Unique ID: 8104312 Medline TA: Curr Eye Res Country: England |
Other Details:
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Languages: eng Pagination: 171-80 Citation Subset: IM |
Affiliation:
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Mitake Eye Clinic, and Department of Biochemistry, School of Medicine, Iwate Medical University, Morioka, Japan. mitake@eins.rnac.ne.jp |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Line Corneal Neovascularization / pathology, prevention & control* Disease Models, Animal Down-Regulation Enzyme-Linked Immunosorbent Assay Gene Silencing / drug effects Humans Microscopy, Fluorescence Pigment Epithelium of Eye / metabolism RNA Interference RNA, Double-Stranded RNA, Messenger / metabolism* RNA, Small Interfering / pharmacology* Rats Rats, Wistar Reverse Transcriptase Polymerase Chain Reaction Transfection Vascular Endothelial Growth Factor A / genetics*, metabolism |
| Chemical | |
Reg. No./Substance:
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0/RNA, Double-Stranded; 0/RNA, Messenger; 0/RNA, Small Interfering; 0/VEGFA protein, human; 0/Vascular Endothelial Growth Factor A |
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