Document Detail


Inhibition of nuclear entry of HPV16 pseudovirus-packaged DNA by an anti-HPV16 L2 neutralizing antibody.
MedLine Citation:
PMID:  20684966     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Rabbit anti-HPV16 L2 serum (anti-P56/75) neutralizes multiple oncogenic human papillomaviruses (HPVs). We inoculated HeLa cells with HPV16 pseudovirus (16PV) and with anti-P56/75-bound 16PV (16PV-Ab). Both 16PV and 16PV-Ab attached equally well to the cell surface. However, the cell-attached L1 protein of 16PV became trypsin-resistant after incubation at 37°C, whereas approximately 20% of the cell-attached 16PV-Ab L1 remained trypsin-sensitive. Confocal microscopy of HeLa cells inoculated with 16PV revealed packaged DNA in the nucleus at 22h after inoculation; however, nuclear DNA was not detected in cells inoculated with 16PV-Ab. Electron microscopy of HeLa cells inoculated with 16PV showed particles located in multivesicular bodies, lamellar bodies, and the cytosol after 4h; no cytosolic particles were detected after inoculation with 16PV-Ab. These data suggest that anti-P56/75 inhibits HPV infection partly by blocking viral entry and primarily by blocking the transport of the viral genome to the nucleus.
Authors:
Yoshiyuki Ishii; Keiko Tanaka; Kazunari Kondo; Takamasa Takeuchi; Seiichiro Mori; Tadahito Kanda
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-08-04
Journal Detail:
Title:  Virology     Volume:  406     ISSN:  1096-0341     ISO Abbreviation:  Virology     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2010-09-13     Completed Date:  2010-10-05     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0110674     Medline TA:  Virology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  181-8     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Elsevier Inc. All rights reserved.
Affiliation:
Pathogen Genomics Center, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo 162-8640, Japan. yishii@nih.go.jp
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MeSH Terms
Descriptor/Qualifier:
Active Transport, Cell Nucleus / drug effects
Animals
Antibodies, Neutralizing / pharmacology*
Antibodies, Viral / pharmacology*
Capsid Proteins / immunology*
Cell Line
Cell Nucleus / immunology,  metabolism,  virology*
DNA, Viral / genetics,  metabolism*
Hela Cells
Human papillomavirus 16 / genetics,  immunology,  physiology*
Humans
Oncogene Proteins, Viral / immunology*
Papillomavirus Infections / immunology,  metabolism,  virology*
Rabbits
Virus Assembly / drug effects
Chemical
Reg. No./Substance:
0/Antibodies, Neutralizing; 0/Antibodies, Viral; 0/Capsid Proteins; 0/DNA, Viral; 0/L2 protein, Human papillomavirus type 16; 0/Oncogene Proteins, Viral

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