Document Detail


Inhibition of nitric oxide synthesis augments pulmonary oedema in isolated perfused rabbit lung.
MedLine Citation:
PMID:  11064616     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The role of nitric oxide (NO) in precipitating pulmonary oedema in acute lung injury remains unclear. We have investigated the mechanism of involvement of NO in the maintenance of liquid balance in the isolated rabbit lung. Thirty pairs of lungs were perfused with colloid for up to 6 h, during which pulmonary vascular resistance (PVR) and capillary pressure (PCP) were measured frequently, and time to gain 5 g in weight (t5) was recorded. Four protocols with different perfusate additives were studied: (i) none (control, n = 11); (ii) 10 mmol NG-nitro-L-arginine methyl ester (L-NAME) (n = 6); (iii) 10 mmol L-NAME with 100 mumol lodoxamide, an inhibitor of mast cell degranulation (n = 7); (iv) 10 mmol L-NAME with 10 mumol 8-bromo-3',5'-cyclic guanosine monophosphate (8Br-cGMP), an analogue of cGMP that may reduce vascular permeability by relaxing contractile elements in endothelial cells (n = 6). Neither PVR nor PCP differed between protocols. L-NAME markedly reduced t5 from 248 (27) min (mean (SEM)) in protocol (i) to 144 (5) min in protocol (ii) (P < 0.05). Both lodoxamide (t5 = 178 (7) min) and 8Br-cGMP (t5 = 204 (10) min) substantially corrected the effect of L-NAME (P < 0.005). Results suggest that maintenance of a low permeability by NO may involve mast cell stabilization and endothelial cell relaxation.
Authors:
A L Mundy; K L Dorrington
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  British journal of anaesthesia     Volume:  85     ISSN:  0007-0912     ISO Abbreviation:  Br J Anaesth     Publication Date:  2000 Oct 
Date Detail:
Created Date:  2000-11-09     Completed Date:  2000-11-09     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372541     Medline TA:  Br J Anaesth     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  570-6     Citation Subset:  IM    
Affiliation:
University Laboratory of Physiology, Oxford, UK.
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MeSH Terms
Descriptor/Qualifier:
Animals
Carbon Dioxide / blood
Enzyme Inhibitors / pharmacology
Hydrogen-Ion Concentration
Male
NG-Nitroarginine Methyl Ester / pharmacology
Nitric Oxide / biosynthesis,  physiology*
Nitric Oxide Synthase / antagonists & inhibitors
Organ Culture Techniques
Oxygen / blood
Partial Pressure
Pulmonary Edema / physiopathology*
Rabbits
Vascular Resistance / drug effects
Weight Gain / drug effects
Chemical
Reg. No./Substance:
0/Enzyme Inhibitors; 10102-43-9/Nitric Oxide; 124-38-9/Carbon Dioxide; 50903-99-6/NG-Nitroarginine Methyl Ester; 7782-44-7/Oxygen; EC 1.14.13.39/Nitric Oxide Synthase

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