| INHIBITION OF NF-κB SENSITISES ANTERIOR PITUITARY CELLS TO TNF-α- AND LPS-INDUCED APOPTOSIS. | |
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MedLine Citation:
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PMID: 21564349 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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NF-κB, an important proinflammatory factor, is a crucial regulator of cell survival. Both lipopolysaccharide (LPS) and tumor necrosis factor-α (TNF-α) activate NF-κB signalling. Oestrogens were shown to suppress NF-κB activation. Oestrogens exert a sensitizing action to proapoptotic stimuli such as LPS and TNF-α in anterior pituitary cells. In the present study, we show by Western blot that 17β-oestradiol (E(2) ) decreases TNF-α-induced NF-κB/p65 and p50 nuclear translocation in primary cultures of anterior pituitary cells from ovariectomized (OVX) rats. Also, in vivo administration of E(2) decreases LPS-induced NF-κB/p65 and p50 nuclear translocation. To investigate whether the inhibition of NF-κB pathway sensitises anterior pituitary cells to proapoptotic stimuli we used an inhibitor of NF-κB activity, BAY 11-7082 (BAY). BAY, at a concentration that fails to induce apoptosis, has permissive action on TNF-α-induced apoptosis of lactotrophs and somatotrophs from OVX rats, as assessed by TUNEL assay. Pharmacological inhibition of NF-κB signalling enhances E(2) -sensitising effect to TNF-α-induced apoptosis in lactotrophs but not in somatotrophs. In vivo administration of BAY allowed LPS-induced apoptosis in anterior pituitary cells from OVX rats (determined by FACS). Also, LPS-induced expression of Bcl-xL in pituitaries of OVX rats is decreased by E(2) administration. Our results show that inhibition of the NF-κB signalling pathway sensitised anterior pituitary cells to the proapoptotic action of LPS and TNF-α. Since E(2) inhibits LPS- and TNF-α-activated NF-κB nuclear translocation, the present study suggests that E(2) sensitises anterior pituitary cells to TNF-α- and LPS-induced apoptosis by inhibiting NF-κB activity. |
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Authors:
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Guadalupe Eijo; Sandra Zárate; Gabriela Jaita; Jimena Ferraris; María Laura Magri; Verónica Zaldivar; Daniela Radl; Valeria Boti; Daniel Pisera; Adriana Seilicovich |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-5-12 |
Journal Detail:
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Title: Journal of neuroendocrinology Volume: - ISSN: 1365-2826 ISO Abbreviation: - Publication Date: 2011 May |
Date Detail:
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Created Date: 2011-5-13 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8913461 Medline TA: J Neuroendocrinol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Journal of Neuroendocrinology © 2011 Blackwell Publishing. |
Affiliation:
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Instituto de Investigaciones en Reproducción, Facultad de Medicina, Universidad de Buenos Aires, Paraguay 2155, Buenos Aires (ABG1121), Argentina. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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