Document Detail


Inhibition of myoblast migration by prostacyclin is associated with enhanced cell fusion.
MedLine Citation:
PMID:  17488951     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Satellite cells are stem cells that are critical for the formation and growth of skeletal muscle during myogenesis. To differentiate and fuse, proliferating satellite cells or myoblasts must migrate and establish stable cell-cell contacts. However, the factors that regulate myoblast migration and fusion are not understood completely. We have identified PGI2 as a novel regulator of myogenesis in vitro. PGI2 is a member of the family of prostaglandins (PG), autocrine/paracrine signaling molecules synthesized via the cyclooxygenase-1 and -2 pathways. Primary mouse muscle cells both secrete PGI2 and express the PGI2 receptor, IP, at various stages of myogenesis. Using genetic and pharmacological approaches, we show that PGI2 is a negative regulator of myoblast migration that also enhances cell fusion. Thus, PGI2 may act as a "brake" on migrating cells to facilitate cell-cell contact and fusion. Together, our results highlight the importance of the balance between positive and negative regulators in cell migration and myogenesis. This work may have implications for migration of other populations of adult stem cells and/or cells that undergo fusion.
Authors:
Brenda A Bondesen; Kristen A Jones; Wayne C Glasgow; Grace K Pavlath
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2007-05-08
Journal Detail:
Title:  FASEB journal : official publication of the Federation of American Societies for Experimental Biology     Volume:  21     ISSN:  1530-6860     ISO Abbreviation:  FASEB J.     Publication Date:  2007 Oct 
Date Detail:
Created Date:  2007-10-01     Completed Date:  2008-01-07     Revised Date:  2012-02-15    
Medline Journal Info:
Nlm Unique ID:  8804484     Medline TA:  FASEB J     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3338-45     Citation Subset:  IM    
Affiliation:
Emory University School of Medicine, Department of Pharmacology, Atlanta, GA 30322, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antihypertensive Agents / metabolism
Cell Differentiation
Cell Fusion*
Cell Movement / physiology*
Cells, Cultured
Cytochrome P-450 Enzyme System / metabolism
Epoprostenol / agonists,  metabolism*
Intramolecular Oxidoreductases / metabolism
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
Mice, Knockout
Muscle Development / physiology*
Myoblasts / cytology,  physiology*
Receptors, Epoprostenol / genetics,  metabolism
Signal Transduction / physiology
Grant Support
ID/Acronym/Agency:
AR051372/AR/NIAMS NIH HHS; AR052730/AR/NIAMS NIH HHS; AR47314/AR/NIAMS NIH HHS; AR48884/AR/NIAMS NIH HHS
Chemical
Reg. No./Substance:
0/Antihypertensive Agents; 0/Receptors, Epoprostenol; 35121-78-9/Epoprostenol; 9035-51-2/Cytochrome P-450 Enzyme System; EC 5.3.-/Intramolecular Oxidoreductases; EC 5.3.99.4/prostacyclin synthetase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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