| Inhibition of mouse hepatocyte apoptosis via anti-Fas ribozyme. | |
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MedLine Citation:
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PMID: 15300908 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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AIM: To investigate the effects of anti-Fas ribozyme on Fas expression and apoptosis in primary cultured mouse hepatocytes. METHODS: Mouse hepatocytes were isolated by using collagenase irrigation. A hammerhead ribozyme targeting the Fas mRNA was constructed, and transfected into mouse hepatocytes via Effectene. Then Fas expression in mouse hepatocytes was detected by RT-PCR and western blotting. After being treated with anti-Fas antibody (JO2), hepatocytes viability was measured with MTT assay. Caspase-3 proteolytic activity was detected, and cell apoptosis was measured according to Annexin V-FITC apoptosis detection kit. RESULTS: Fas expressed in primary mouse hepatocytes. Fas expression in hepatocytes transfected with anti-Fas ribozyme was decreased remarkably and correlated with the resistance to Fas-mediated apoptosis as determined by flow cytometry and caspase-3 proteolytic activity. CONCLUSION: Anti-Fas ribozyme can remarkably decrease the Fas expression in mouse hepatocytes, thus inhibit Fas-mediated apoptosis in hepatocytes. It is suggested that anti-Fas ribozyme could significantly increase the resistance of transplanted hepatocytes to apoptosis and improve the survival of transplanted hepatocytes. |
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Authors:
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Min Zhang; Wei He; Fang Liu; Ping Zou; Juan Xiao; Zhao-Dong Zhong; Zhong-Bo Hu |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: World journal of gastroenterology : WJG Volume: 10 ISSN: 1007-9327 ISO Abbreviation: World J. Gastroenterol. Publication Date: 2004 Sep |
Date Detail:
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Created Date: 2004-08-09 Completed Date: 2004-09-20 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 100883448 Medline TA: World J Gastroenterol Country: China |
Other Details:
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Languages: eng Pagination: 2567-70 Citation Subset: IM |
Copyright Information:
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Copyright 2004 The WJG Press ISSN |
Affiliation:
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Institute of Hematology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei Province, China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antigens, CD95 / genetics* Apoptosis / physiology* Caspase 3 Caspases / metabolism Cell Survival / physiology Cells, Cultured Gene Expression Hepatocytes / cytology* Mice RNA, Catalytic / genetics* Transfection |
| Chemical | |
Reg. No./Substance:
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0/Antigens, CD95; 0/RNA, Catalytic; 0/hammerhead ribozyme; EC 3.4.22.-/Casp3 protein, mouse; EC 3.4.22.-/Caspase 3; EC 3.4.22.-/Caspases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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