Document Detail

Inhibition of mouse hepatocyte apoptosis via anti-Fas ribozyme.
MedLine Citation:
PMID:  15300908     Owner:  NLM     Status:  MEDLINE    
AIM: To investigate the effects of anti-Fas ribozyme on Fas expression and apoptosis in primary cultured mouse hepatocytes. METHODS: Mouse hepatocytes were isolated by using collagenase irrigation. A hammerhead ribozyme targeting the Fas mRNA was constructed, and transfected into mouse hepatocytes via Effectene. Then Fas expression in mouse hepatocytes was detected by RT-PCR and western blotting. After being treated with anti-Fas antibody (JO2), hepatocytes viability was measured with MTT assay. Caspase-3 proteolytic activity was detected, and cell apoptosis was measured according to Annexin V-FITC apoptosis detection kit. RESULTS: Fas expressed in primary mouse hepatocytes. Fas expression in hepatocytes transfected with anti-Fas ribozyme was decreased remarkably and correlated with the resistance to Fas-mediated apoptosis as determined by flow cytometry and caspase-3 proteolytic activity. CONCLUSION: Anti-Fas ribozyme can remarkably decrease the Fas expression in mouse hepatocytes, thus inhibit Fas-mediated apoptosis in hepatocytes. It is suggested that anti-Fas ribozyme could significantly increase the resistance of transplanted hepatocytes to apoptosis and improve the survival of transplanted hepatocytes.
Min Zhang; Wei He; Fang Liu; Ping Zou; Juan Xiao; Zhao-Dong Zhong; Zhong-Bo Hu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  World journal of gastroenterology : WJG     Volume:  10     ISSN:  1007-9327     ISO Abbreviation:  World J. Gastroenterol.     Publication Date:  2004 Sep 
Date Detail:
Created Date:  2004-08-09     Completed Date:  2004-09-20     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  100883448     Medline TA:  World J Gastroenterol     Country:  China    
Other Details:
Languages:  eng     Pagination:  2567-70     Citation Subset:  IM    
Copyright Information:
Copyright 2004 The WJG Press ISSN
Institute of Hematology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei Province, China.
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MeSH Terms
Antigens, CD95 / genetics*
Apoptosis / physiology*
Caspase 3
Caspases / metabolism
Cell Survival / physiology
Cells, Cultured
Gene Expression
Hepatocytes / cytology*
RNA, Catalytic / genetics*
Reg. No./Substance:
0/Antigens, CD95; 0/RNA, Catalytic; 0/hammerhead ribozyme; EC 3.4.22.-/Casp3 protein, mouse; EC 3.4.22.-/Caspase 3; EC 3.4.22.-/Caspases

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