Document Detail


Inhibition of migration and invasion of colorectal cancer cells via deletion of Rac1 with RNA interference.
MedLine Citation:
PMID:  19009236     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cell migration and invasion are triggered by a number of chemoattractants that stimulate intracellular signaling pathways through regulating reorganization of the actin cytoskeleton. Rac1, an intracellular signal transducer, regulates a variety of cell functions, including the organization of the cytoskeleton, cell migration, and invasion. However, currently, very little is known about the roles of Rac1 in the cytoskeleton formation and invasion of human colorectal cancer cells. In our study, Rac1-shRNA was used to silence the Rac1 to reduce its expression specifically in Lovo cells. Our studies showed that RNA interference-mediated deletion of Rac1 strongly inhibited lamellipodia formation, cell migration, and invasion of Lovo cells in vitro. The deletion of Rac1 can serve as an alterative therapy to inhibit the invasion and metastasis of colorectal cancer cells.
Authors:
Shi-Yi Zhao; Yan Sun; Zhuo-Sheng Lai; Qing-Zhen Nan; Kang Li; Zhen-Shu Zhang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-11-15
Journal Detail:
Title:  Molecular and cellular biochemistry     Volume:  322     ISSN:  1573-4919     ISO Abbreviation:  Mol. Cell. Biochem.     Publication Date:  2009 Feb 
Date Detail:
Created Date:  2009-01-27     Completed Date:  2009-08-05     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0364456     Medline TA:  Mol Cell Biochem     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  179-84     Citation Subset:  IM    
Affiliation:
Department of Gastroenterology, Nanfang Hospital, Southern Medical University, Guangzhou Road 1838, Guangzhou, 510515, Guangdong, China.
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MeSH Terms
Descriptor/Qualifier:
Cell Adhesion
Cell Line, Tumor
Cell Movement*
Colorectal Neoplasms / genetics*,  metabolism,  pathology
Cytoskeleton / metabolism
Humans
Microscopy, Confocal
Pseudopodia / metabolism
RNA Interference*
Sequence Deletion
Transfection
rac1 GTP-Binding Protein / antagonists & inhibitors*,  genetics,  metabolism
Chemical
Reg. No./Substance:
EC 3.6.5.2/rac1 GTP-Binding Protein

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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