| Inhibition of microsomal calcium sequestration causes an impairment of initiation of protein synthesis in perfused rat liver. | |
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MedLine Citation:
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PMID: 2059198 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The present study examined the effect of 2,5-di-(tert-butyl)-hydroquinone (tBuHQ), an inhibitor of liver microsomal calcium sequestration, on initiation of protein synthesis in perfused rat liver. Perfusion of livers with a concentration of tBuHQ previously shown to completely inhibit microsomal calcium sequestration in isolated hepatocytes caused a 50% inhibition of protein synthesis. The inhibition was characterized by an increase in liver content of free ribosomal particles and a decrease in polysomes indicating that peptide-chain initiation was slowed relative to elongation. Furthermore, the inhibition was associated with a 7.5-fold increase in the proportion of the alpha-subunit of eukaryotic initiation factor 2 (eIF-2) present in the phosphorylated form and a reduction in the activity of eukaryotic initiation factor 2B (eIF-2B) to 37% of the control value. The results suggest that protein synthesis in rat liver is regulated directly by changes in intracellular calcium concentration through a mechanism involving modulation of the phosphorylation state of eIF-2 alpha. |
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Authors:
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S R Kimball; L S Jefferson |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Biochemical and biophysical research communications Volume: 177 ISSN: 0006-291X ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 1991 Jun |
Date Detail:
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Created Date: 1991-07-31 Completed Date: 1991-07-31 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1082-6 Citation Subset: IM |
Affiliation:
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Department of Cellular and Molecular Physiology, College of Medicine, Pennsylvania State University, Hershey Medical Center 17033. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Calcium / metabolism* Eukaryotic Initiation Factor-2 / metabolism* Hydroquinones / pharmacology* Kinetics Liver / drug effects, metabolism* Male Microsomes, Liver / drug effects, metabolism* Perfusion Phosphorylation Protein Biosynthesis* Protein Synthesis Inhibitors / pharmacology* Rats Rats, Inbred Strains |
| Grant Support | |
ID/Acronym/Agency:
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DK13499/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Eukaryotic Initiation Factor-2; 0/Hydroquinones; 0/Protein Synthesis Inhibitors; 1948-33-0/2-tert-butylhydroquinone; 7440-70-2/Calcium |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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