Document Detail

Inhibition of metabolic cooperation by cigarette smoke condensate and its fractions in V-79 Chinese hamster lung fibroblasts.
MedLine Citation:
PMID:  6577428     Owner:  NLM     Status:  MEDLINE    
This study was performed to determine the usefulness of the intercellular metabolic cooperation assay for analysis of a complex mixture and to compare the results obtained with previously conducted in vivo tumor promoter assays. One hundred 2R1 cigarettes were smoked according to Federal Trade Commission guidelines and the resulting condensate was separated into a water/methanol-soluble fraction (which was further partitioned into acidic and basic components) and an organic solvent-soluble fraction (which was then chromatographed on silicic acid with petroleum ether, benzene/petroleum ether, benzene, ether, and methanol). The following fractions were positive in the metabolic cooperation assay (in decreasing order of activity): organic solvent-soluble, acidic, whole condensate, and water/methanol-soluble fractions as well as the ether, benzene, and benzene/petroleum ether eluates. The basic fraction and the petroleum ether and methanol eluates were negative. In general, the metabolic cooperation assay results were comparable to previously published results obtained on mouse skin.
T G Hartman; J D Rosen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  80     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  1983 Sep 
Date Detail:
Created Date:  1983-10-08     Completed Date:  1983-10-08     Revised Date:  2010-09-13    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  5305-9     Citation Subset:  IM    
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MeSH Terms
Cell Line
Cell Survival / drug effects
Drug Evaluation, Preclinical / methods
Hypoxanthine Phosphoribosyltransferase / genetics
Smoke / adverse effects*
Reg. No./Substance:
0/Carcinogens; 0/Smoke; EC Phosphoribosyltransferase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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