Document Detail

Inhibition of mammalian muscle differentiation by regeneration blastema extract of Sternopygus macrurus.
MedLine Citation:
PMID:  18816861     Owner:  NLM     Status:  MEDLINE    
Tissue regeneration through stem cell activation and/or cell dedifferentiation is widely distributed across the animal kingdom. By comparison, regeneration in mammals is poor and this may reflect a limited dedifferentiation potential of mature cells. Because mammalian myotubes can dedifferentiate in the presence of newt blastema extract, the present study tested the dedifferentiation induction capability of the blastema from the teleost Sternopygus macrurus (SmBE). Our in vitro data showed that SmBE did not induce cell cycle reentry of myonuclei in myotubes. Instead, SmBE caused myotubes to detach and time-lapse imaging analyses characterized the cellular events before their detachment. Furthermore, SmBE enhanced myoblast proliferation and reversibly inhibited their differentiation. These data suggest the presence of protein factors in SmBE that regulate mammalian muscle physiology and differentiation, but do not support the conservation of a dedifferentiation induction capability by the blastema of S. macrurus.
Hyun-Jung Kim; Eric Archer; Norma Escobedo; Stephen J Tapscott; Graciela A Unguez
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Developmental dynamics : an official publication of the American Association of Anatomists     Volume:  237     ISSN:  1058-8388     ISO Abbreviation:  Dev. Dyn.     Publication Date:  2008 Oct 
Date Detail:
Created Date:  2008-10-02     Completed Date:  2008-11-26     Revised Date:  2013-11-26    
Medline Journal Info:
Nlm Unique ID:  9201927     Medline TA:  Dev Dyn     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2830-43     Citation Subset:  IM    
Copyright Information:
Copyright (c) 2008 Wiley-Liss, Inc.
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MeSH Terms
Biological Markers
Cell Differentiation*
Cell Extracts
Cell Nucleus
Cell Proliferation
Muscle Fibers, Skeletal / cytology
Muscles / cytology*
Grant Support
GM007667-30S1/GM/NIGMS NIH HHS; P20 RR016480/RR/NCRR NIH HHS; P20 RR016480-059004/RR/NCRR NIH HHS; R25GM061222/GM/NIGMS NIH HHS; RR16480/RR/NCRR NIH HHS; S06 GM008136/GM/NIGMS NIH HHS; S06 GM008136-310053/GM/NIGMS NIH HHS; S06-GM008136/GM/NIGMS NIH HHS; U56 CA096286/CA/NCI NIH HHS; U56 CA096286-010003/CA/NCI NIH HHS; U56 CA096286-05/CA/NCI NIH HHS; U56-CA96286/CA/NCI NIH HHS
Reg. No./Substance:
0/Biological Markers; 0/Cell Extracts

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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